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过程工程学报 ›› 2018, Vol. 18 ›› Issue (6): 1119-1125.DOI: 10.12034/j.issn.1009-606X.218234

• 中科院过程工程所60周年特邀 • 上一篇    下一篇

磁性Fe3O4@SiO2颗粒结构对其吸附DNA的影响

邢慧芳1,2， 杨良嵘1*， 于杰淼1， 刘会洲1， 余 浩3， 李万波3， 崔水东3   

  1. 1. 中国科学院过程工程研究所绿色过程与工程重点实验室，北京 100190 2. 中国科学院大学化学工程学院，北京 100049 3. 盘古基因生物工程(南京)股份有限公司，江苏 南京 210000
  • 收稿日期:2018-06-28 修回日期:2018-08-10 出版日期:2018-12-22 发布日期:2018-12-19
  • 通讯作者: 邢慧芳 hfxing@ipe.ac.cn
  • 基金资助:
    国家自然科学基金;国家自然科学基金重点项目;国家科技支撑计划;中国科学院青年创新促进会资助项目

Effect of magnetic Fe3O4@SiO2 nanoparticles structure on its adsorption to DNA

Huifang XING1,2,  Liangrong YANG1*,  Jiemiao YU1,  Huizhou LIU1,  Hao YU3,  Wanbo LI3,  Shuidong CUI3   

  1. 1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 2. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China 3. PangoGene BioScience Ltd., Nanjing, Jiangsu 210000, China
  • Received:2018-06-28 Revised:2018-08-10 Online:2018-12-22 Published:2018-12-19

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摘要： 采用共沉淀法和水热法制备了不同结构的超顺磁性Fe3O4@SiO2纳米颗粒，对其进行表征，研究了其吸附DNA的性能及磁分离性能. 结果表明，20?750 nm范围内粒径较大的颗粒与DNA结合时可提供更多单位平面结合位点，使结合的稳定性和结合几率增加，DNA结合量提高. 不同核?壳结构的Fe3O4@SiO2纳米颗粒的磁分离响应时间不同，内核大小相近时，壳层厚度增加会导致颗粒在磁场中受到的磁力与阻力的比值减小，磁响应时间增加，DNA回收率降低. 粒径约为200 nm的Fe3O4@SiO2纳米颗粒用于纯化全血中DNA最好，提取率为95.2%，磁响应时间为10 s.

关键词: 磁性二氧化硅, DNA提取, 颗粒结构, 磁分离

Abstract: DNA purification is considered to be a critical step in biomedical applications such as genetic therapy and clinical diagnosis. These years, the magnetic silica beads are widely used in DNA purification due to the advantages of averting the use of some toxic organic solvents and being easy to auto-magnetic separation. Among them, magnetic Fe3O4@SiO2 nanoparticles (NPs) were widely used because of their stability, monodispersity, selectivity, and high separation efficiency in magnetic field. However, the shape and structure of particles may affect the DNA isolation efficiency, such as the DNA adsorption capacity and recovery rate. It has not yet been fully explored. In this work, a series of Fe3O4 NPs with different core diameters were synthesized firstly by co-precipitation and hydrothermal methods, and then further coated by silica through St?ber method. The resultant Fe3O4@SiO2 NPs were characterized by SEM, TEM, IR and BET, respectively. The DNA adsorption capacity of Fe3O4@SiO2 NPs were studied by UV?Vis, and the magnetic separation properties were also determined by magnetic response time. The results showed that in the range of 20?750 nm of particle size, the larger size particles could provide more unit planar binding sites when combining with DNA, which increased the combination stability and binding probability. Thus, the DNA binding capacity of the particles increased with the increase of particles size. In addition, the magnetic response time of Fe3O4@SiO2 NPs with different core?shell structures were also different. When the size of the core was similar, the thicker SiO2 shell around would weaken the dipole?dipole interactions between particles and reduce particles aggregation, thus the magnetic response time of NPs increased, leading to a low recovery rate within a limited operating time. Comparing the DNA adsorption capacity and recovery efficiency of particles. The Fe3O4@SiO2 NPs around 200 nm was the optimal choice for blood DNA purification, which had high recovery rate (95.2%) in a short magnetic response time (10 s).

Key words: magnetic silica, DNA separation, particle structure effect, magnetic separation