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›› 2005, Vol. 5 ›› Issue (2): 201-204.

• 生化工程专栏 • 上一篇    下一篇

西维因人工抗原的合成新方法

杨耀军,渠桂荣,孟凡涛,马光辉   

  1. 河南师范大学
  • 出版日期:2005-04-20 发布日期:2005-04-20

Novel Synthesis of the Antigen for Carbaryl

YANG Yao-jun,QU Gui-rong,MENG Fan-tao,MA Guang-hui   

  1. College of Chemistry and Environmental Science, Henan Normal University
  • Online:2005-04-20 Published:2005-04-20

摘要: 用对硝基苯氯甲酸酯活化萘酚并与6-氨基己酸反应,合成了完全保留氨基甲酸酯结构的半抗原6-(1-萘氧基甲酰胺基)己酸(CNH),并采用活化酯法与载体蛋白共价连接制备了突出西维因结构特征的抗原. 用正交实验选择活化酯法的最佳实验条件,并进一步详细考察了半抗原与载体蛋白结合率随外界条件的改变,从而制得不同结合比的抗原. 用紫外和红外对制得的抗原进行了鉴定.

关键词: 西维因, 对硝基苯氯甲酸酯, 半抗原, 抗原, 活性酯法

Abstract: A hapten 6-[(1-naphthyloxy) carbonyl] amino hexanoic acid (CNH) completely preserving the carbamate group was synthesized with aminohexanoic acid and 1-naphthol, which was activated with 4-nitrophenyl chloroformate. Then the CNH hapten was covalently attached to carrier protein by utilizing the modified active ester method to synthesize antigen. Some factors influencing synthesis of CNH antigen were studied, and the conditions were optimized through orthogonal design experiments. A series of CNH-BSA antigens of different CNH/BSA mole ratios were synthesized by altering the experimental conditions. The antigens were identified with the UV-VIS and IR analysis.

Key words: carbaryl, 4-nitrophenyl chloroformate, hapten, antigen, active ester method