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›› 2005, Vol. 5 ›› Issue (3): 341-344.

• 生化工程专栏 • 上一篇    下一篇

人血红蛋白分子印迹聚合物的制备及分子识别性能

孙瑞丰,罗晖,于慧敏,沈忠耀   

  1. 清华大学 化学工程系 生物化工研究所
  • 出版日期:2005-06-20 发布日期:2005-06-20

Preparation and Specific Molecular Recognition Properties of Human Hemoglobin Imprinted Polymers

SUN Rui-feng,LUO Hui,YU Hui-min,SHEN Zhong-yao   

  1. Department of Chemical Engineering, Tsinghua University
  • Online:2005-06-20 Published:2005-06-20

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摘要： 以丙烯酰胺和甲基丙烯酸为功能单体制备得到了具有选择性识别能力的人血红蛋白分子印迹聚合物. 该印迹聚合物的分子识别特性来自聚合物的结合基团与印迹分子的功能基团之间的氢键和静电作用，以及印迹孔穴的空间几何选择性. 研究表明，聚合单体中甲基丙烯酸的含量会影响聚合物对血红蛋白的特异结合能力及分子印迹聚合物的吸附容量和洗脱性能.

关键词: 丙烯酰胺, 甲基丙烯酸, 人血红蛋白, 分子印迹聚合物, 分子识别

Abstract: Using acrylamide and methylacrylic acid as functional monomers, human hemoglobin molecular-imprinting-polymers (HHb-MIPs) were successfully prepared. The studies showed that the specific molecular recognition properties of HHb-MIPs were related to both the interactions between the functional groups of polymers and imprinted protein, and geometric complementation of the cavities in the polymers. Further studies showed that the content of methyacrylic acid in the polymers could affect the specific binding properties of MIPs. The supplementation of methylacrylic acid can increase the specific adsorption capacity of the HHb-MIPs on the one hand, but decrease the elution capacity on the other hand.

Key words: acrylamide, methylacrylic acid, human hemoglobin, molecular imprinting polymers, molecular recognition