欢迎访问过程工程学报, 今天是

›› 2006, Vol. 6 ›› Issue (1): 77-81.

• 生化工程专栏 • 上一篇    下一篇

豆粕水解液为氮源细菌厌氧流加发酵生产L-乳酸

丁绍峰,谭天伟   

  1. 北京化工大学生命科学与技术学院北京市生物加工过程重点实验室
  • 出版日期:2006-02-20 发布日期:2006-02-20

Production of L-Lactic Acid by Anaerobic Fed-batch Fermentation by Lactic Acid Bacteria with Soybean Meal Hydrolysate as Nitrogen Source

DING Shao-feng,TAN Tian-wei   

  1. Key lab of Bioprocess of Beijing, Col. Life Sci.& Technol., Beijing Univ. Chem. Technol.
  • Online:2006-02-20 Published:2006-02-20

摘要: 采用细菌厌氧发酵法生产L-乳酸,由实验确定了最佳接种量、发酵温度和pH调节剂,考察了初始葡萄糖浓度对L-乳酸生产的影响,确定初始糖浓度为70~90 g/L时得率、产率、最终生物量分别达到92.68 g/g, 3.17 g/(L×h)和8.5′107 mL-1. 为进一步降低L-乳酸生产成本,以豆粕水解液为氮源代替酵母粉,同时应用流加发酵技术,L-乳酸产量、得率、产率及转化率分别达到155 g/L, 95.5 g/g, 1.64 g/(L×h)和96.9%. 在保证L-乳酸最终浓度的同时可降低生产成本,为进一步工业化奠定了基础.

关键词: L-乳酸, 豆粕水解液, 流加发酵

Abstract: The L-lactic acid production by anaerobic fermentation of lactic acid bacteria was studied. The effects of fermentation conditions such as inoculation, temperature and pH neutralizer on the fermentation of L-lactic acid were determined. When the initial medium containing 70~90 g/L glucose was used, the maximum L-lactic acid yield (92.68%) on total glucose consumed, L-lactic acid productivity [3.17 g/(L×h)] and the cell final biomass (8.5′107 mL-1) were obtained. A new promising nitrogen source, soybean meal hydrolysate (SMH), can replace yeast extract (YE) completely in order to reduce the manufacturing cost of lactic acid. The maximum L-lactic acid concentration (155 g/L) in fed-batch culture was obtained besides L-lactic acid yield, productivity and the rate of glucose consumed were up to 95.5 g/g, 1.64 g/(L×h) and 96.9% respectively.

Key words: L-lactic acid, soybean meal hydrolysate, fed-batch fermentation