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›› 2006, Vol. 6 ›› Issue (2): 277-280.

• 生化工程专栏 • 上一篇    下一篇

普通小球藻异养-光自养串联培养的培养基

李兴武,李元广, 沈国敏,杨东   

  1. 华东理工大学海洋生化工程研究所
  • 出版日期:2006-04-20 发布日期:2006-04-20

Medium for Culturing Chlorella vulgaris with Sequential Heterotrophic-Autotrophic Model

LI Xing-wu,LI Yuan-guang,SHEN Guo-min,YANG Dong   

  1. Institute of Marine Bioprocess Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Inst.Marine Bioprocess Eng., East China Univ. Sci. Technol
  • Online:2006-04-20 Published:2006-04-20

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摘要： 采用单因素实验和均匀实验获得了适合于普通小球藻异养-光自养串联培养的培养基(HA-SK培养基)，其关键是C/N比和保证足够的C, N供应. 采用该培养基在摇瓶中异养-光自养串联培养普通小球藻，异养培养结束时细胞密度达13.17 g/L，经过36 h光自养培养后藻体蛋白质和叶绿素含量分别达49.75%和30.17 mg/g. 用5 L生物反应器和1 L平板光生物反应器串联培养，藻细胞密度最高可达15.36 g/L，藻体蛋白质和叶绿素含量分别达54.78%和31.23 mg/g. 表明采用HA-SK培养基进行异养-光自养串联培养可实现普通小球藻的高密度高品质培养.

关键词: 异养-光自养串联培养, 普通小球藻, 培养基, 蛋白质含量, 叶绿素含量

Abstract: The medium (HA-SK medium) for culturing Chlorella vulgaris with sequential heterotrophic-autotrophic model was optimized by using the one-at-a-time strategy and uniformity experimental design. When Chlorella vulgaris was cultured with HA-SK medium in 250 mL flask, the cell density reached 13.17 g/L at the end of heterotrophic cultivation, the cellular protein and chlorophyll content were 49.75% and 30.17 mg/g after 36 h autotrophic cultivation, which were increased by 134%, 69.8% and 68.7% respectively comparing with those obtained in an initial medium. As Chlorella vulgaris was cultured with HA-SK medium in sequential 5 L bioreactor and 1 L plat photo-bioreactor system, the cell density reached 15.36 g/L at the end of heterotrophic cultivation, the cellular protein and chlorophyll content were 54.78% and 31.23 mg/g after 35 h autotrophic cultivation. The results show that the cultivation of Chlorella vulgaris with high cell density and high quality can be realized successfully by sequential heterotrophic-autotrophic model with HA-SK medium.

Key words: sequential heterotrophic-autotrophic cultivation, Chlorella vulgaris, medium, protein content, chlorophyll content