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›› 2007, Vol. 7 ›› Issue (3): 531-535.

• 过程与工艺 • 上一篇    下一篇

反胶束法制备纳米氧化锌及其在葡萄糖生物传感器中的应用

郭伟玲,任湘菱,唐芳琼,陈东,张琳,刘奉岭   

  1. 中国科学院理化技术研究所
  • 出版日期:2007-06-20 发布日期:2007-06-20

Preparation of ZnO Nanoparticles in a Reverse Micellar System and Their Application in Glucose Biosensor

GUO Wei-ling,REN Xiang-ling,TANG Fang-qiong,CHEN Dong,ZHANG Lin,LIU Feng-ling   

  1. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences
  • Online:2007-06-20 Published:2007-06-20

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摘要： 采用琥珀酸-2-乙基己基磺酸钠/环己烷反胶束体系制备纳米ZnO，并以此ZnO为载体固定葡萄糖氧化酶，用戊二醛交联制作成葡萄糖氧化酶电极. 实验结果表明，此酶电极表现出对葡萄糖溶液浓度的优良响应，线性范围在1′10-5~3′10-3 mol/L，响应灵敏度约为6 mA/(cm2×mmol/L)，表观米氏常数为2.57 mmol/L. 还研究了温度和溶液pH值对电极电流响应的影响.

关键词: 反胶束, 纳米ZnO, 葡萄糖生物传感器, 酶固定

Abstract: ZnO nanoparticles are produced in sodium-bis-(2-ethylhexyl ) sulfosuccinate (AOT)/cyclohexane reverse micelle system and then used to immobilize glucose oxidase (GOD) to prepare enzyme electrode of biosensor. The experimental results show that ZnO nanoparticles can significantly enhance the catalytic activity of the immobilization enzyme, and the electrode has a very good response to the concentration of glucose solution. The linear range for glucose determination is from 1′10-5 to 3′10-3 mol/L, with a high sensitivity [6 mA/(cm2×mmol/L)] and the apparent Michaelis-Menten constant of 2.57 mmol/L. In addition, the effects of pH value and temperature of glucose solution on the amperometric response of the electrode are also investigated.

Key words: reverse micelles, ZnO nanoparticles, glucose biosensor, immobilization enzyme