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›› 2007, Vol. 7 ›› Issue (4): 778-781.

• 生化工程专栏 • 上一篇    下一篇

利用水解酶提取文冠果油脂

刘苗苗,王晓东,赵兵,王玉春   

  1. 中国科学院过程工程研究所生化工程国家重点实验室
  • 出版日期:2007-08-20 发布日期:2007-08-20

Extraction of Xanthoceras sorbifolia Bunge Oil with Hydrolysis Enzymes

LIU Miao-miao,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun   

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences
  • Online:2007-08-20 Published:2007-08-20

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被引次数

27

摘要： 采用3种酶对文冠果种仁进行水酶法提油，其中Alcalase 2.4 L蛋白酶的效果最好. 在Alcalase 2.4 L蛋白酶用量为0.02 mL/g的条件下，通过单因素实验和Box-Behnken中心组合实验，应用SAS软件分析得出水酶法提取文冠果油脂的最佳工艺条件为：温度55℃, pH 9, 固液比1 g:6 mL, 水解时间4 h，在此条件下油脂提取率和蛋白水解度分别达到78.67%和9.15%.

关键词: 文冠果, 水解酶, 油脂提取, 蛋白水解

Abstract: Three commercial enzymes were used for extraction of oil from Xanthoceras sorbifolia Bunge seeds. The results showed that Alcalase 2.4 L was the most effective hydrolysis enzyme. The main factors influencing the oil extraction rate and degree of protein hydrolysis were analyzed by response surface methodology. Under the fixed ratio of enzyme to seeds (0.02 mL/g), the optimal parameters were temperature 55℃, pH=9, the ratio of solid to water 1:6 (g/mL), and enzyme incubation time 4 h. Under these conditions, the oil extraction rate and degree of protein hydrolysis reached 78.67% and 9.15% respectively.

Key words: Xanthoceras sorbifolia Bunge, hydrolysis enzyme, oil extraction, protein hydrolysis