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›› 2007, Vol. 7 ›› Issue (6): 1197-1201.

• 生化工程专栏 • 上一篇    下一篇

亚心形扁藻培养基的优化及光合特性

刘远,陈兆安,陆洪斌,刘长发,金美芳,郭祯,张卫   

  1. 中国科学院大连化学物理研究所海洋生物产品工程组
  • 出版日期:2007-12-20 发布日期:2007-12-20

Optimization of Culture Medium and Photosynthetic Characteristics of Platymonas subcordiformis

LIU Yuan,CHEN Zhao-an,LU Homg-bin,LIU Chang-fa,JIN Mei-fang,GUO Zhen,ZHANG Wei   

  1. Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics
  • Online:2007-12-20 Published:2007-12-20

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被引次数

11

摘要： 以均匀实验设计方法优化亚心形扁藻(Platymonas subcordiformis)培养基，采用U10*(108)均匀设计实验考察NO3--N, PO43--P, 缓冲体系(Tris-HAc)三因素对亚心形扁藻生长的影响，同时也考察了与其光合作用相关的PSII光化学活性、藻液pH、集光色素3个指标的变化. 结果表明，在NO3--N 9.5 mmol/L, PO43--P 0.34 mmol/L, Tris-HAc 6.7 mmol/L的优化条件下，亚心形扁藻生长迅速，15 d藻细胞密度增殖到(8.28±0.51)×106 mL-1. Tris-HAc体系能够维系藻细胞在最优pH条件下增殖，高浓度氮源利于PSII光化学活性保持恒定和集光色素的积累，从而提高了光能利用效率，促进藻细胞生长.

关键词: 均匀设计, 亚心形扁藻, 光能利用效率, 培养基

Abstract: Uniform design method was applied for culture medium optimization of a marine microalga, Platymonas subcordiformis. Using the uniform design table U10*(108), three key medium ingredients (nitrate-N, phosphate-P, and Tris-HAc buffer) were chosen as optimizing factors. Their effects on the pH variation, photochemical activity of photosystem II (PSII) and light harvesting pigment of microalga cultures were examined. The optimum medium ingredients were obtained as follows: NO3--N 9.5 mmol/L, PO43--P 0.34 mmol/L, Tris-HAc buffer 6.7 mmol/L, under which P. subcordiformis cells could grow efficiently, reaching a density of (8.28±0.51)×106 mL-1 within 15 d. It was found that the buffer Tris-HAc maintained a stable pH value, and high NO3--N level improved the photosynthetic efficiency and growth rate of cells.

Key words: uniform design, Platymonas subcordiformis, photosynthetic efficiency, culture medium