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›› 2008, Vol. 8 ›› Issue (1): 115-119.

• 生化工程专栏 • 上一篇    下一篇

超声波对新疆紫草悬浮培养细胞生长和紫草素合成的影响

葛锋,陈朝银,王晓东,赵兵,王玉春   

  1. 昆明理工大学生物与化学工程学院
  • 出版日期:2008-02-20 发布日期:2008-02-20

Effects of Ultrasonic Wave on Cell Growth and Shikonin Biosynthesis in Suspension Culture of Arnebia euchroma

GE Feng,CHEN Chao-yin,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun   

  1. Faculty of Biological and Chemical Engineering, Kunming University of Science and Technology
  • Online:2008-02-20 Published:2008-02-20

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被引次数

33

摘要： 探讨了超声波功率、超声波处理时间对新疆紫草细胞生长和紫草素合成的影响. 研究结果表明，超声波对悬浮培养的新疆紫草细胞生长有促进作用，低功率长时间或高功率短时间的超声处理对细胞生长比较有利. 在优化条件下(200 W超声处理1 min)，培养结束时的生物量比对照提高61%. 超声波也可以提高新疆紫草悬浮培养细胞的紫草素含量和产量，接种后即进行超声波处理，超声波功率密度为39.9 mW/cm3、超声时间为3 min时，细胞紫草素含量和产量最高，达到2.72%和294 mg/L，分别比对照组提高了64%和135%. 超声波是通过提高细胞苯丙氨酸解氨酶的活力来强化紫草素的生物合成途径.

关键词: 超声波, 新疆紫草, 紫草素, 细胞培养

Abstract: The effects of ultrasonic wave power and treatment time on the cell growth and shikonin biosynthesis of Arnebia euchroma were investigated. The results showed that the stimulation of ultrasonic wave promoted A. euchroma cell growth in suspension culture. Low power density and long time treatment or high power density and short time treatment by ultrasonic wave was favorable to the cell growth. The cell biomass productivity was 61% higher than that in control by the end of the culture period under the optimal conditions of 200 W ultrasonic wave treatment for 1 min. The shikonin content and shikonin production in suspension culture of A. euchroma cells were enhanced by ultrasonic stimulation as well. When the ultrasonic stimulation was carried out after inoculation, the ultrasonic power density was 39.9 mW/cm3 and the stimulation time was 3 min, the shikonin content and shikonin production gained the highest results (2.72%, 294 mg/L), which were 64% and 135% higher than those obtained in control, respectively. The strengthening of shikonin biosynthesis may be due to increasing the activity of phenylalanine ammonia lyase caused by ultrasonic stimulation.

Key words: ultrasonic wave, Arnebia euchroma, shikonin, cell culture