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›› 2012, Vol. 12 ›› Issue (1): 125-130.

• 生化工程专栏 • 上一篇    下一篇

黄鳝血清转铁蛋白的提取及表征

王博文 任天瑞 董亚明   

  1. 上海师范大学生命与环境科学学院 上海师范大学 生命与环境科学学院 上海师范大学 生命与环境科学学院
  • 收稿日期:2011-06-03 修回日期:2011-12-20 出版日期:2012-02-20 发布日期:2012-02-20
  • 通讯作者: 任天瑞

Extraction and Characterization of Serotransferrin from Monopterus albus

WANG Bo-wen REN Tian-rui DONG Ya-ming   

  1. College of Life and Environment Science, Shanghai Normal University College of Life and Environment Sciences, Shanghai Normal University College of Life and Environment Sciences, Shanghai Normal University
  • Received:2011-06-03 Revised:2011-12-20 Online:2012-02-20 Published:2012-02-20
  • Contact: REN Tian-rui

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摘要： 对从黄鳝血液中提取的蛋白层析纯化后进行生物质谱测试，确定为血清转铁蛋白. 利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、原子力显微镜、圆二色谱法对蛋白的分子量、表面形貌、二级结构进行了表征. 结果表明，该转铁蛋白分子量为74 kDa，含量为0.750 mg/mL，含a-螺旋29.8%，b-折叠6.6%.

关键词: 黄鳝, 转铁蛋白, 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳, 生物质谱, 原子力显微镜

Abstract: The crude extraction of serum protein in Monopterus albus obtained by salting was purified by ion exchange chromatography. By means of biological mass spectrometry, the protein was identified as serotransferrin, which was the serum transferrin. Its molecular weight, surface morphology and secondary structure were characterized by SDS-PAGE, atomic force microscopy and circular dichroism, respectively. The results show that the molecular weight of serotransferrin is 74 kDa, its content 0.750 mg/mL, including a-helix 29.8% and b-fold 6.6%.

Key words: Monopterus albus, transferrin, SDS-PAGE, biological mass spectrum, atomic force microscopy

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