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›› 2011, Vol. 11 ›› Issue (2): 324-328.

• 生化工程专栏 • 上一篇    下一篇

水相中酿酒酵母催化3-氯-1-苯丙酮的不对称还原反应

杨锦 方世银 石贤爱   

  1. 福州大学生物科学与工程学院 福州大学生物科学与工程学院 福州大学生物科学与工程学院
  • 收稿日期:2011-01-05 修回日期:2011-03-21 出版日期:2011-04-20 发布日期:2011-04-20
  • 通讯作者: 石贤爱

Asymmetric Reduction of 3-Chloropropiophenone with Saccharomyces cerevisiae in Aqueous System

YANG Jin FANG Shi-yin SHI Xian-ai   

  1. College of Biological Science and Engineering, Fuzhou University College of Biological Science and Engineering, Fuzhou University College of biological science and technologe , Fuzhou University
  • Received:2011-01-05 Revised:2011-03-21 Online:2011-04-20 Published:2011-04-20
  • Contact: SHI Xian-ai

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摘要： 实验考察了水相体系中酿酒酵母细胞对3-氯-1-苯丙酮不对称还原的影响. 结果表明，该反应的优势产物为(S)-3-氯-1-苯丙醇，且反应是由单一酶或具有相同催化特性的同工酶催化的. 在实验浓度范围内，该反应不存在底物抑制和产物抑制，但产物对细胞存在毒性而导致细胞失去催化能力，影响产物得率的提高. 当反应体系中Tween-80浓度为2.0 mmol/L, b-环糊精浓度为5.0 mmol/L时，得率从对照组的64.7%分别提高到73.8%和70.6%. 说明同时具有包结作用和改善细胞膜渗透性的Tween-80提高产物得率的效果优于仅具有包结作用的b-环糊精.

关键词: 酿酒酵母, 3-氯-1-苯丙酮, Tween-80, 包结作用, 不对称还原

Abstract: Asymmetric reduction of 3-chloropropiophenone with Saccharomyces cerevisiae in aqueous system was studied. The results showed that the preference product configuration was (S)-3-chloro-1-phenylpropanol, and the reduction was catalyzed by a single reductase or several isozymes with the same catalytic characteristics. In the concentration range investigated, neither substrate nor product inhibitions were found, but the toxicity of product which induced the cell inactivation prevented the yield from elevation. The proper concentration of inclusion complexes in the reaction system assisted to improve the yield, for example, when the concentration of Tween-80 and b-cyclodextrin was 2.0 and 5.0 mmol/L, the yields would be increased from the control of 64.7% to 73.8% and 70.6%, respectively. The additives with the abilities of inclusion and improvement of cell membrane permeability would take better effect on the yield than those only with the ability of inclusion.

Key words: Saccharomyces cerevisiae, 3-chloropropiophenone, Tween-80, inclusion, asymmetric reduction

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