有机介质处理酿酒酵母对其催化不对称还原反应性能的影响

doi:10.12034/j.issn.1009-606X.217217

过程工程学报 ›› 2017, Vol. 17 ›› Issue (6): 1281-1286.DOI: 10.12034/j.issn.1009-606X.217217

有机介质处理酿酒酵母对其催化不对称还原反应性能的影响

李锋^1,2，方世银¹，石贤爱^1,3*

1．福州大学药物生物技术与工程研究所，福建福州 350116；2. 福州大学化学学院，福建福州 350116； 3. 福建省医疗器械与医药技术重点实验室，福建福州 350116

收稿日期:2017-04-17 修回日期:2017-05-31 出版日期:2017-12-20 发布日期:2017-12-05
通讯作者: 李锋 lifeng9676@aliyun.com
基金资助:
福建省自然科学基金;福建省教育厅科技项目

Effects of Organic Media Treatment Saccharomyces cerevisiae on Its Catalytic Performance to Asymmetric Reduction Reaction

Feng LI^1,2, Shiyin FANG¹, Xian¢ai SHI^1,3*

1. Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou, Fujian 350116, China; 2. College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China; 3. Fujian Key Laboratory of Medical Instrument & Pharmaceutical Technology, Fuzhou, Fujian 350116, China

Received:2017-04-17 Revised:2017-05-31 Online:2017-12-20 Published:2017-12-05

摘要/Abstract

摘要： 采用不同有机介质处理酿酒酵母细胞，改变细胞膜的通透性、流动性及完整性等膜生物学特性，考察有机介质处理对酵母细胞催化2-辛酮不对称还原反应性能的影响. 结果表明，在实验条件下，当细胞内核酸和蛋白泄露值达0.057和0.046，或细胞的碘化丙啶(PI)吸收因子达1.157时，反应产率显著降低；有机介质处理的细胞膜的各向异性值为未处理细胞的111.7%时，细胞丧失催化2-辛酮不对称还原反应的能力；膜受损的细胞占比达到或超过41.8%时，反应产率明显降低. 在细胞膜完整的前提下，细胞膜通透性和流动性处于合适范围才能使酵母细胞催化2-辛酮不对称还原反应的性能良好.

关键词: 酿酒酵母, 不对称还原, 细胞膜特性, 有机介质

Abstract: Different organic media were used to induce changes of cell membrane properties, i.e. the membrane permeability, mobility and integrity. The effect of organic media treatment on the asymmetric reduction of 2-octanone catalyzed by Saccharomyces cerevisiae cells was investigated. The results showed that the reaction yield was significantly decreased when the leakage values of nucleic acid and protein reached 0.057 and 0.046 respectively, or the propidium iodide (PI) uptake factor reached 1.157. When the anisotropy value of cell membrane labeled with diphenylhexatriene (DPH) reached 111.7% of the control group, the cells lost the ability of catalyzing asymmetric reduction. When the ratio of cell with damaged membrane integrity reached 41.8%, the reaction yield was significantly reduced. In the premise of maintaining the integrity of cell membrane, cell membrane permeability and mobility in the appropriate range make Saccharomyces cerevisiae cells show good performance on catalyzing 2-octanone asymmetric reduction reaction.

Key words: Saccharomyces cerevisiae, asymmetric reduction, cell membrane properties, organic media

李锋方世银石贤爱. 有机介质处理酿酒酵母对其催化不对称还原反应性能的影响[J]. 过程工程学报, 2017, 17(6): 1281-1286.

Feng LI Shiyin FANG Xianai SHI. Effects of Organic Media Treatment Saccharomyces cerevisiae on Its Catalytic Performance to Asymmetric Reduction Reaction[J]. Chin. J. Process Eng., 2017, 17(6): 1281-1286.

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有机介质处理酿酒酵母对其催化不对称还原反应性能的影响

Effects of Organic Media Treatment Saccharomyces cerevisiae on Its Catalytic Performance to Asymmetric Reduction Reaction

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