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›› 2011, Vol. 11 ›› Issue (5): 828-833.

• 生化工程专栏 • 上一篇    下一篇

Penicillium expansum脂肪酶选择性拆分外消旋萘普生

唐良华 蔡志雄 苏敏 祝铃 周琼 吕博彦   

  1. 福建师范大学生命科学学院 福建师范大学生命科学学院 福建师范大学生命科学学院 福建师范大学生命科学学院 福建师范大学生命科学学院 福建师范大学生命科学学院
  • 收稿日期:2011-05-13 修回日期:2011-07-19 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 唐良华

Enantioselective Resolution of Racemic Naproxen by a Lipase from Penicillium expansum

TANG Liang-hua CAI Zhi-xiong SU Min ZHU Ling ZHOU Qiong LU Bo-yan   

  1. College of Life Sciences, Fujian Normal University College of Life Sciences, Fujian Normal University College of Life Sciences, Fujian Normal University College of Life Sciences, Fujian Normal University College of Life Sciences, Fujian Normal University College of Life Sciences, Fujian Normal University
  • Received:2011-05-13 Revised:2011-07-19 Online:2011-10-20 Published:2011-10-20
  • Contact: TANG Liang-hua

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摘要： 为了实现固定化扩展青霉TS414(Penicillium expansum TS414)脂肪酶在有机相中对外消旋萘普生的高效拆分，实验考察了水分、温度、有机溶剂、酶浓度、醇结构和醇浓度对酶促拆分反应的影响，确立了优化的酯化反应条件为：异辛烷为溶剂，外消旋萘普生2.15 mmol/L，正丙醇34.3 mmol/L，固定化酶量12 g/L，水0.05%(j), 40℃恒温摇床中200 r/min反应100 h. 在此条件下，酯化拆分反应的转化率为48.3%. 结果表明，固定化Penicillium expansum脂肪酶是一种较为理想的用于外消旋萘普生拆分的工具酶.

关键词: 脂肪酶, 萘普生, 拆分

Abstract: In order to resolute racemic naproxen with high efficiency by the immobilized Penicillium expansum TS414 lipase in organic solvent, the factors affecting the resolution of (R, S)-naproxen by lipase-catalyzed esterification reaction were investigated, including water concentration, temperature, organic solvent, enzyme concentration, structure and concentration of alcohol. And experiments were designed to optimize the conditions of esterification reaction. Under the optimal condition (isooctane as solvent, 2.15 mmol/L ibuprofen, 12 g/L lipase, 34.3 mmol/L 1-propanol, 0.05%(j) water, 200 r/min, 40℃ for 100 h), the conversion rate could reach 48.3%. Thus, Penicillium expansum TS414 lipase could be used as a perfect tool enzyme with high enantioselectivity and had a great potential for commercial applications in the resolution of racemic naproxen and other non-steroidal anti-inflammatory drugs of 2-aryl propionic Acids.

Key words: lipase, naproxen, resolution

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