Chin. J. Process Eng. ›› 2016, Vol. 16 ›› Issue (3): 494-499.DOI: 10.12034/j.issn.1009-606X.215389
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SUI Chun-hong WANG Zhao-yi WEI Yu-qing WANG Cheng
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隋春红 王昭懿 韦雨清 王程
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Abstract: The carboxyl groups of poly(acrylic acid) (PAA)/polyvinyl alcohol (PVA) microfibrous membranes were activated by using dicyclohexyl-carbodiimide and N-hydroxysuccinimide. The free amines of glucoamylase were reacted with the activated carboxyls to form ester bond, which immobilized the enzyme onto the electrospun PAA/PVA microfiber by covalent bonds. The enzymatic reaction of the immobilized glucoamylase was investigated. The results showed that glucoamylase was attached on the surface of electrospun PAA/PVA composite microfibrous membrane, and the diameter of the fiber was about 435 nm. The maximum reaction velocity (Vmax) and michaelis constant (Km) of the immobilized glucoamylase were 5.77 g/(L×h) and 10.45 g/L, respectively. The optimum pH range and temperature of the immobilized enzyme were similar to those of the free enzyme. Immobilized glucoamylase had higher storage stability. It retained 62% of the original activity in 12 d, as compared to about 40% for the free enzyme. The immobilized glucoamylase could be used for 10 times while retaining 40.5% of its activity.
Key words: electrospinning, immobilized enzyme, active ester, poly(acrylic acid)
摘要: 以高压静电纺聚丙烯酸(PAA)/聚乙烯醇(PVA)纤维膜为载体,以羟基琥珀酰亚胺和二环己基碳二亚胺为基团偶联剂和活化剂,采用活化酯法固定葡萄糖淀粉酶. 结果表明,葡萄糖淀粉酶可固定于高压静电纺PAA/PVA纤维膜表面,固载后纤维直径约为435 nm,固定化酶的最大反应速率为5.77 g/(L×h),米氏常数Km=10.45 g/L;游离酶与固定化酶的最适pH值和温度基本相同,贮存12 d固定酶活力为新鲜酶的62%,游离酶活力约为新鲜酶的40%;固定化酶重复使用10次仍能保持40.5%活性.
关键词: 静电纺丝, 固定化酶, 活化酯, 聚丙烯酸
CLC Number:
O631
SUI Chun-hong WANG Zhao-yi WEI Yu-qing WANG Cheng. Immobilization of Glucoamylase onto Electrospun PAA/PVA Microfibrous Membrane by Active Ester Method[J]. Chin. J. Process Eng., 2016, 16(3): 494-499.
隋春红 王昭懿 韦雨清 王程. 以静电纺PAA/PVA纤维膜为载体活化酯法固定葡萄糖淀粉酶[J]. 过程工程学报, 2016, 16(3): 494-499.
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URL: https://www.jproeng.com/EN/10.12034/j.issn.1009-606X.215389
https://www.jproeng.com/EN/Y2016/V16/I3/494