›› 2006, Vol. 6 ›› Issue (2): 281-284.
• 生化工程专栏 • Previous Articles Next Articles
WU Yuan-yuan,BAO Yong-ming,LI Xiao-hui,XIU Zhi-long
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吴媛媛,包永明,李晓晖,修志龙
Abstract: Yolk immunoglobulin (IgY) was obtained by injecting chloramphenicol (CAP) bound to bovine serum albumin (BSA) into the hens. A primary purification was conducted according to several kinds of methods, such as PEG and ammonium sulphate precipitations. A relatively high purity and specific activity of IgY were obtained by an optimized method of capralic acid-ammonium sulphate precipitation, and a plenty of phospholipid was well separated, which led to benefiting to store for a long time. Using bis (3-aminopropyl) amine as a spacer between the gel and the CAP ligand, a separation material was synthesized for affinity chromatography to purify the specific IgY against CAP. The specific IgY was about 3.3% in the total IgY. The antibody activity was increased by 10 times after purification by affinity chromatography.
Key words: chloramphenicol, yolk immunoglobulin, affinity chromatography
摘要: 将合成的氯霉素(CAP)与牛血清白蛋白(BSA)的偶联物(CAP-BSA)作为免疫抗原注射到母鸡体内得到氯霉素卵黄抗体(IgY). 比较了不同的提取方法,用优化的辛酸-硫酸铵沉淀法从卵黄液中初步提取卵黄抗体. 选用3,3¢-二氨丙基亚胺(DADPA)作为亲和凝胶与配基氯霉素之间的"间隔臂",合成了分离纯化抗氯霉素特异性IgY的亲和层析柱. 将IgY粗品经过亲和层析柱,得到的特异性IgY的抗体活性提高了10倍,收率约为3.3%.
关键词: 氯霉素, 卵黄抗体, 亲和层析
WU Yuan-yuan;BAO Yong-ming;LI Xiao-hui;XIU Zhi-long. Preparation and Purification of Immunoglobulin Yolk against Choramphenicol[J]. , 2006, 6(2): 281-284.
吴媛媛;包永明;李晓晖;修志龙. 抗氯霉素卵黄抗体的制备及其分离纯化[J]. , 2006, 6(2): 281-284.
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