›› 2007, Vol. 7 ›› Issue (2): 395-398.
• 生化工程专栏 • Previous Articles Next Articles
GAO Yang,NIE Kai-li,WANG Fang,TAN Tian-wei
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Published:
高阳,聂开立,王芳,谭天伟
Abstract: Candida sp. 99-125 lipase was entrapped in organic-inorganic sol-gel powder prepared by polymerization of tetramethoxysilane (TMOS) and propyltrimethoxysilane (PTMS). PEG (MW=20000) and HCl were used as stabilizing additive and catalyst during the sol-gel process, respectively. The optimal preparation conditions were studied, which are PTMS/TMOS molar ratio at 4:1, water/silane molar ratio 20, enzyme loading 3.71% (w) of silane precursor and hydrolysis time of silane precursor for 35 min. The specific activity of immobilized lipase was 2.02 times higher than that of non-immobilized lipase under the optimal water content in esterification reaction system. The esterification activity of immobilized lipase retained 59.1% of the initial activity after 2 h incubation under 100℃, and the activity of immobilized lipase remained almost unchanged after 8 consecutive batches of each 12 h reaction.
Key words: sol-gel, entrapment, lipase
摘要: 利用正硅酸甲酯(TMOS)和丙基三甲氧基硅烷(PTMS)为复合硅源,以PEG(MW=20000)为稳定剂,以HCl为催化剂,经过溶胶-凝胶过程包埋假丝酵母99-125脂肪酶. 研究得到最适的固定化条件为:PTMS与TMOS的摩尔比4: 1, R值(水与硅源的摩尔比)20, 给酶量(酶占硅源的质量百分数)3.71%, PEG与酶的质量比(1~1.5):1, 硅源水解时间35 min. 在该条件下,固定化脂肪酶的最高酯化活力是游离酶最高酯化活力的2.02倍. 固定化脂肪酶在100℃保温2 h后酶活仍维持为59.1%,固定化酶催化特定酯化反应,经过8批连续反应96 h后酶活维持不变.
关键词: 溶胶-凝胶, 固定化, 脂肪酶
GAO Yang;NIE Kai-li;WANG Fang;TAN Tian-wei. Immobilization of Lipase by Sol-Gel Entrapment[J]. , 2007, 7(2): 395-398.
高阳;聂开立;王芳;谭天伟. 溶胶-凝胶法固定化Candida sp.99-125脂肪酶[J]. , 2007, 7(2): 395-398.
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