Abstract: Immobilization of lipase on macroporous poly(glycidyl methacrylate-co-divinylbenzene) was investigated and followed by the optimization of immobilizing conditions. The optimized results were achieved with the 1:1 mass ratio of lipase to the carrier, coupling temperature between 20~25℃, and immobilizing time of 1.5 h. The optimum pH and temperature of immobilized lipase were 8.5 and 40℃, respectively. The activity of immobilized lipase remained 94% of the origin after storage at 4℃ for 7 d. The experimental results showed that the thermal stability and operational stability were improved in comparison with free lipase.

Key words: macroporous carrier, lipase, immobilized lipase

摘要： 用自制大孔载体固定化脂肪酶，对固定化条件进行了优化，比较了固定化酶与游离酶的酶学参数. 结果表明，酶粉与载体质量比为1:1、固定化温度在20~25℃之间、固定化时间1.5 h的条件下，所得固定化酶的酶活最高. 固定化酶的最适pH为8.5，最适温度为40℃，其热稳定性、操作稳定性都比游离酶高，4℃下保存7 d后，酶活仍剩余94%.

关键词: 大孔载体, 脂肪酶, 固定化酶