Abstract: The optimal conditions of microcystis aeruginosa cultured in a photobioreactor were established. The effects of light intensity and Fe3+ concentration on Microcystis aeruginosa growth and microcystin production in the photobioreactor were studied. The Fe3+ concentration in the culture medium changed between 0~500 mmol/L. The results showed that the cells growth and microcystin-LR production were both affected by changing Fe3+ concentration and light intensity. Biomass and chlorophyll were limited when Fe3+ concentration was below 0.1 μmol/L and reached the maximum when Fe3+ concentration was 100 μmol/L. Then they both dropped down when Fe3+ concentration was further increased. The maximum production of microcystin-LR was obtained at 10 mmol/L Fe3+ concentration. The effect of light intensity on microcystin-LR production was also studied under the optimal Fe3+ concentration. The results showed that the optimal light intensity for microcystin-LR production was 30 mmol/(m2×s).

Key words: Fe3+ concentration, photobioreactor, Microcystis aeruginosa, microcystin-LR, light intensity

摘要： 在2.5 L光生物反应器中考察了光强、通气量等对铜绿微囊藻生长的影响，确定了最佳生长条件. 在此条件下考察不同Fe3+浓度(0~500 mmol/L)对铜绿微囊藻的生长、叶绿素及微囊藻毒素(MC-LR)含量的影响，同时考察光照强度对铜绿微囊藻产毒素的影响. 实验结果表明，在低铁(Fe3+<0.1 mmol/L)、缺铁(Fe3+<0.01 mmol/L)及高铁(Fe3+>100 mmol/L)环境下，微囊藻生长、叶绿素及毒素合成均受到抑制. 生物量和叶绿素在Fe3+浓度为100 mmol/L时含量最高，毒素在Fe3+浓度为10 mmol/L、光强为30 mmol/(m2×s)时含量最大.

关键词: Fe3+浓度, 光反应器, 铜绿微囊藻, 微囊藻毒素, 光照强度