Abstract: The bioleaching of nickel-molybdenum sulfide ore with thermophilic and acidophilic lithotrph, Sulfolobus metallicus, with shaking flasks was studied. The result shows that the leaching rate of Ni with Sulfolobus metallicus was all more than 90%, but that in the groups without Sulfolobus metallicus was 77.64%. The leaching rates of Ni and Mo in the groups with tamed Sulfolobus metallicus were 94.7% and 70.2% respectively, and those in groups with untamed Sulfolobus metallicus 93.1% and 68.4%. The leaching rates of Ni and Mo in the groups at pH 2 were 100% and 66.97% respectively. All of Ni in the groups with the sizes under -0.048 mm and -0.077 mm was leached out, while the leaching rates of Mo were 68.4% and 64.5%. The leaching rates of Ni and Mo in the groups at 5 g/L ore content were 100% and 87.29% respectively. In the groups with Fe3+ 0.5 g/L added and contrast group without Sulfolobus metallicus, the leaching rates of Ni were respectively 92.8% and 76.6%, and Mo 52.56% and 49.34%. The leaching rates of Ni and Mo in the groups with Sulfolobus metallicus were 93.17% and 73.52% respectively, while just 67.34% of Ni and 38.36% of Mo were leached out in the groups with Thiobacillus ferrooxidans.

Key words: Sulfolobus metallicus, Thiobacillus ferrooxidans, nickel-molybdenum sulfide ore, strain taming, shaking flask leaching

摘要： 对金属硫叶菌浸出镍钼硫化矿进行了研究. 结果表明，有菌组镍的浸出率均在90%以上，而无菌组为77.64%；驯化菌比非驯化菌的浸出率高，前者镍和钼的浸出率分别为94.7%和70.2%，后者为93.1%和68.4%；pH为2时浸出效果最佳，镍浸出率达100%，钼浸出率为66.97%；粒径<0.048 mm和<0.077 mm的浸样镍浸出率均达到100%，钼浸出率分别为68.4%和64.5%；低矿浆浓度比高矿浆浓度的浸出率高，5 g/L矿浆镍和钼的浸出率分别达100%和87.29%；在无菌条件下，浸样添加0.5 g/L Fe3+和对照组的镍浸出率分别为92.8%和76.6%，钼浸出率为52.56%和49.34%；嗜热菌(金属硫叶菌)比常温菌(氧化亚铁硫杆菌)的浸出率高，前者镍钼浸出率分别为93.17%和73.52%，后者为67.34%和38.36%.

关键词: 金属硫叶菌, 氧化亚铁硫杆菌, 镍钼硫化矿, 菌株驯化, 摇瓶浸出