Abstract: Five different non-ionic surfactants, BSA, Tween-20, Tween-80, PEG-4000 and PEG-6000, were evaluated for their ability to enhance simultaneous saccharification and fermentation (SSF) of wheat straw. The results indicated that all non-ionic surfactants tested improved the SSF of wheat straw, and Tween-20 was the most effective. Addition of Tween-20 decreased the enzyme loading while retaining the same ethanol concentration. The ethanol concentration of 18.7 g/L was achieved at a substrate concentration of 50 g/L at 37℃ and pH 5.0 using cellulase mixture (25 FPU/g of substrate for Celluclast 1.5 l and 15 IU/g for Novozym 188) for 72 h. Compared with the reference run without Tween-20, the ethanol concentration was increased by 14.0% whereas the residence time reduced by 12 h.

Key words: wheat straw, surfactant, simultaneous saccharification and fermentation, ethanol

摘要： 研究了5种非离子型表面活性剂(BSA, Tween-20, Tween-80, PEG-4000, PEG-6000)促进麦草同步糖化发酵的效果. 结果表明，5种表面活性剂均能促进麦草同步糖化发酵，以Tween-20效果最为显著. 反应体系中添加Tween-20可降低酶用量而保持乙醇浓度基本相同. 在pH 5.0、温度37℃、底物浓度50 g/L及Celluclast 1.5 l用量25 FPU/g、Novozym 188用量15 IU/g的反应体系中，添加0.03 g/g Tween-20，反应72 h，乙醇浓度达到18.7 g/L，比未添加表面活性剂的体系提高了14.0%，反应时间缩短了12 h.

关键词: 麦草, 表面活性剂, 同步糖化发酵, 乙醇