Abstract: Polyethyleneimine (PEI) was grafted onto the surface of silica gel particles via the coupling effect of 3-methacryloxypropyl trimethoxysilane (MPS), and the grafted particle PEI/SiO2 was prepared. Then, the molecular imprint polyethyleneimine on the surface of silica gel, MIP-PEI/SiO2, was prepared with bilirubin as a template molecule and ethylene glycol diglycidyl ether (EGDE) as crosslinking agent by using novel molecular surface imprinting technique. Both static and dynamic methods were adopted to study the identification properties of MIP-PEI/SiO2 for bilirubin. The experimental results show that MIP-PEI/SiO2 has higher selectivity ability and excellent combination property for bilirubin. Besides, MIP-PEI/SiO2 has excellent elution property, with the mixed solvent of EDTA and NaOH as the eluent, the desorption ratios of bilirubin reach 99.39% in 11 bed volumes.

Key words: polyethyleneimine, silica gel, molecular imprint, surface imprint, bilirubin

摘要： 以g-(甲基丙烯酰氧)丙基三甲氧基硅烷(MPS)为媒介，将功能大分子聚乙烯亚胺(PEI)偶合接枝到硅胶微粒表面，形成了接枝微粒PEI/SiO2. 以胆红素为模板分子，乙二醇二缩水甘油醚(EGDE)为交联剂，采用分子表面印迹技术，制备了胆红素分子表面印迹材料MIP-PEI/SiO2，采用静态与动态两种方法研究了MIP-PEI/SiO2对胆红素的结合特性. 结果表明，印迹材料MIP-PEI/SiO2对胆红素分子具有特异的识别选择性与优良的结合亲和性，也具有优良的解吸性能，以EDTA与NaOH混合溶液作为洗脱液，11个床体积内解吸率可达99.39%.

关键词: 聚乙烯亚胺, 硅胶, 分子印迹, 表面印迹, 胆红素