Abstract: Molecularly imprinted polymer was synthesized with Rifampicin (RFP) as template molecule, methacrylic acid as functional monomers and ethylene dimethacrylate as crosslinker. The polymer was investigated in equilibrium binding experiment and Scatchard analysis to evaluate the binding characteristics and recognition mechanism of RFP molecular imprinted polymer in aqueous solution. Scatchard analysis showed that two classes of binding sites were produced in the polymer matrix under the studied concentration. Their dissociation constant (Kd) and apparent maximum binding capacity (Qmax) were Kd1=31.5 mg/mL, Qmax1=23.34 mg/g for high affinity binding sites and Kd2=9.22 mg/mL, Qmax2=12.86 mg/g for low affinity binding sites. The binding experiments of different substrates showed that the imprinted polymers possessed tailor-made recognition sides for RFP.

Key words: molecularly imprinted polymer, Rifampicin, molecular recognition, selectivity

摘要： 以利福平为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂，制备了利福平分子印迹聚合物. 采用平衡结合方法和Scatchard模型评价了该聚合物的结合特性，考察了其吸附行为. 结果表明，利福平分子印迹聚合物中形成了两类不同的结合位点，得到高亲和力结合位点的离解常数和最大表观结合量分别为31.5 mg/mL和23.34 mg/g，低亲和力结合位点的离解常数和最大表观结合量分别为9.22 mg/mL和12.86 mg/g. 实验结果显示，利福平分子印迹聚合物对利福平呈现出了高的选择吸附特性.

关键词: 分子印迹聚合物, 利福平, 识别机理, 选择性