Abstract: The effects of reaction temperature, pH value, initial substrate concentration, reaction duration, cell concentration and forms on biotransformation reaction for preparation of (R)-1-phenyl-1,2-ethanediol were studied with 2-hydroxyacetophenone and sodium formate as substrates and the whole cells of E. coli Rosetta/pETDuet-rcr-fdh as catalyst .The results showed that when the concentration of 2-hydroxyacetophenone was 6 g/L, (R)-1-phenyl-1,2-ethanediol was produced with high optical purity of 98.37% enantiomeric excess and yield of 79.14% at the conditions of temperature 35℃, pH 7.0, reaction duration 36 h and wet cell concentration 10%(w). Further research showed that the biotransformation efficiency could be well improved by addition of Zn2+. In the presence of Zn2+ with a final concentration of 5 mmol/L, the enantiomeric excess and yield reached 100% and 86.3%, respectively.

Key words: recombinant E. coli strain, biotransformation, (R)-1-phenyl-1, 2-ethanediol, enantiomeric excess, yield

摘要： 以重组菌E. coli Rosetta/pETDuet-rcr-fdh全细胞为催化剂，2-羟基苯乙酮和甲酸钠为双底物，研究了生物催化反应体系中温度、pH值、底物2-羟基苯乙酮的初始浓度、反应时间、细胞浓度和状态等对产物(R)-苯基乙二醇生成效率的影响规律. 结果表明，在温度35℃和pH 7.0、底物初始浓度6 g/L、时间36 h、湿细胞浓度10%(w)的条件下，产物的光学纯度高达98.37% e.e.，得率达79.14%. 在上述反应体系中添加5 mmol/L ZnSO4后，重组菌催化的不对称还原反应效率显著提高，产物(R)-苯基乙二醇光学纯度达到100% e.e.，得率高达86.3%.

关键词: 重组大肠杆菌, 生物催化, (R)-苯基乙二醇, 光学纯度, 得率