Abstract: The culture medium optimization of Bacillus mucilaginosus PM13 strain mutated for soil improvement was studied. The effects of the medium composition on the growth of PM13 strain and viscosity of the broth were investigated. The results of single-factor, orthogonal and uniform experiments showed that the varieties of carbon and nitrogen sources affected the growth of PM13 strain and viscosity of the broth significantly. And the ingredients of the optimum culture medium for PM13 growth were (g/L): molasses 3.67, starch 1.83, soybean powder 7.0, CaCO3 8.5, K2HPO4 2.0, MgSO4×7H2O 1.4 and NaCl 0.2. With this culture medium, the production of spores reached 2.2×108 cfu/mL and the fluid consistency coefficient of the broth was markedly decreased to 0.028 Pa×sn, which was suitable for the large-scale fermentation in conventional bioreactors.

Key words: Bacillus mucilaginosus, culture medium, spore production, viscosity

摘要： 对胶质芽孢杆菌PM13菌株进行了培养基成分的优化，考察了培养基各组分对菌体生长和发酵液粘度的影响. 由单因素、正交和均匀实验结果可知，碳源和氮源种类的影响最显著，优化的培养基配方为(g/L)：糖蜜3.67，淀粉5.5，豆粕粉7.0, CaCO3 8.5, K2HPO4 2.0, MgSO4×7H2O 1.4, NaCl 0.2. 在此配方下，胶质芽孢杆菌芽孢生成量达2.2×108 cfu/mL，且发酵液稠度系数显著降低到0.028 Pa×sn，可采用常规生物反应器进行规模化发酵.

关键词: 胶质芽孢杆菌, 培养基成分, 芽孢生成量, 粘度