Abstract: A two-step process involving hydrophobic interaction chromatography and hydrophilic interaction chromatography was developed to purify chemically synthetic oxytocin out of economic and environmental consideration. First, Nylon 6 hydrophobic interaction chromatography was performed using water as mobile phase for desalting and enrichment. And Superose 12 hydrophilic interaction chromatography was performed for further purification. By this two-step purification process, the purity of oxytocin was increased up to 93.5% (5.96 folds purification) with final recovery rate of 50.1%, which was comparable with the results obtained from traditional two-step RPLC purification process with C18 medium. This two-step purification process consumed less organic solvent, which meant lower cost. Nylon 6 and Superose 12 media were chemically stable under extreme pH cleaning conditions and easily reproduced.

Key words: polycaprolactam, agarose gel, hydrophobic interaction chromatography, hydrophilic interaction liquid chromatography, oxytocin, purification

摘要： 将疏水相互作用色谱与亲水相互作用色谱结合，建立了用于化学合成缩宫素粗品分离纯化的两步纯化工艺. 先以纯水为流动相，采用Nylon 6疏水相互作用色谱对粗品进行脱盐与富集，后以Superose 12亲水相互作用色谱进行进一步纯化，两步纯化后，峰面积纯度达93.5%，提高了5.96倍，总回收率为50.1%，与用C18为介质的传统两步反相色谱纯化的结果相当. 本方法有机溶剂用量仅为传统反相色谱的1/7，且两种介质都能耐受极端pH值清洗条件，重复利用率高.

关键词: 聚己内酰胺, 琼脂糖凝胶, 疏水相互作用色谱, 亲水相互作用色谱, 缩宫素, 纯化