Abstract: The strain of Klebseilla pneumoniae was exposed under dielectric barrier discharge plasma at air atmospheric pressure. The physical parameters of plasma discharge and optimum conditions of mutagenesis were established. The exposed cell suspension was acclimatized repeatedly under different concentrations of substrate and product, respectively, and single colony was selected by 96-hole plate. A mutant with improved 1,3-propanediol (1,3-PD) production was obtained and designated as Kp-M2. Compared with wild type, the 1,3-PD concentration was increased by 43% in batch fermentation. The kinetic models of Kp-M2 and wild type in batch fermentation were proposed based on the Logistic and Luedeking-Piret equations of cell growth, product formation and substrate consumption. The maximum specific growth rate, formation coefficient of 1,3-PD coupling cell growth and yield coefficient of 1,3-PD on glycerol in Kp-M2 were significantly higher than those of wild type.

Key words: Klebsiellla pneumoniae, mutagenesis, 3-propanediol, fermentation, kinetics

摘要： 以肺炎克雷伯氏菌为出发菌株，利用大气压介质阻挡放电等离子体诱变，确立等离子体诱变的物理参数和最佳诱变条件，分别以不同浓度的底物和产物筛选培养基对诱变菌液进行驯化，并结合96孔板筛选单菌落，获得一株高产1,3-丙二醇的突变菌株，命名为Kp-M2，其间歇发酵1,3-丙二醇产量比野生菌提高43%. 基于Logistic和Luedeking-Piret方程，得到了描述Kp-M2和野生菌间歇发酵的动力学模型及参数，诱变菌的最大比生长速率、与菌体生长偶联的产物生成系数、产物对底物得率均明显高于野生菌.

关键词: 克雷伯氏菌, 诱变, 3-丙二醇, 发酵, 动力学