Abstract: A new type chitosan-b-cyclodextrin copolymer was prepared with the chitosan and b-cyclodextrin as materials, sodium periodate as oxidant, epichlorohydrin as cross-linker using inverse suspension method. Its structure was characterized by FT-IR and SEM. Its adsorption and desorption performance to puerarin, and reusability were investigated. The results showed that the optimal conditions were: adsorption time 4 h, temperature 35℃, pH=7 and initial concentration of puerarin 0.5 mg/mL, the maximum adsorption of puerarin on chitosan-b-cyclodextrin was 52.43 mg/g, its elution rate of puerarin 89.69%, and it showed good reusability.

Key words: chitosan-cyclodextrin cross-linked copolymer, puerarin, adsorption, epichlorohydrin

摘要： 以壳聚糖与b-环糊精为原料、高碘酸钠为氧化剂、环氧氯丙烷为交联剂，用反相悬浮法制备新型壳聚糖/b-环糊精，用红外光谱和电镜扫描对其进行结构表征，研究其对葛根素的吸附性、洗脱性及重复使用性. 结果表明，最佳吸附条件为：吸附时间4 h，温度35℃，pH=7，葛根素初始浓度0.5 mg/mL，壳聚糖/环糊精对葛根素的吸附量达52.43 mg/g，洗脱率达89.69%，且表现出较好的重复使用性.

关键词: 壳聚糖/环糊精交联聚合物, 葛根素, 吸附, 环氧氯丙烷