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›› 2012, Vol. 12 ›› Issue (6): 1002-1007.

• 生化工程专栏 • Previous Articles     Next Articles

Cloning and Transcriptional Expression Analysis of ARE Gene in Rhodosporidium toruloides

ZHANG Guo-ling OURA Takahiro DU Wei LIU De-hua KAJIWARA Susum   

  1. Institute of Applied Chemistry，Department of Chemical Engineering, Tsinghua University Department of Life Science, Tokyo Institute of Technologytment of Chemical Engineering, Tsinghua University Department of Chemical Engineering, Tsinghua University Department of Chemical Engineering，Tsinghua University Department of Life Science，Tokyo Institute of Technology
  • Received:2012-10-16 Revised:2012-11-21 Online:2012-12-20 Published:2012-12-20
  • Contact: DU Wei

圆红冬孢酵母中ARE基因的克隆与表达水平分析

张国玲 大浦隆宏 杜伟 刘德华 梶原将   

  1. 清华大学化学工程系应化所 东京工业大学生命理工科 清华大学化工系应用化学所 清华大学化学工程系 东京工业大学生命理工
  • 通讯作者: 杜伟

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Abstract: The ARE gene of Rhodosporidium toruloides was cloned and its transcriptional expression level analyzed. 2624 bp DNA sequence of ARE gene with 6 introns was clarified. The open reading frame was 2028 bp coding 675 amino acids. The transcriptional expression of ARE gene in the cells cultivated in nitrogen-limited medium increased with culture time. It was supposed that the transcriptional expression of ARE gene was related to lipid accumulation in R. toruloides.

Key words: Rhodosporidium toruloides, triacylglycerol, ARE gene, transcriptional expression

摘要： 克隆了圆红冬孢酵母R. toruloides中的ARE基因并对转录水平进行了初步分析. 结果表明，克隆得到的ARE基因的DNA序列共2624 bp，含6个内含子，开放阅读框2028 bp，编码675个氨基酸. 在限氮培养基中培养的R. toruloides酵母细胞中，ARE基因的转录表达水平随培养时间增加而提高，推测在酵母R. toruloides中ARE基因可能与油脂积累有关.

关键词: 圆红冬孢酵母, 甘油三酯, ARE基因, 转录表达

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