Abstract: A moderate thermophilic consortium was enriched from acid mine drainage and coal mine wastewater samples collected in China. After adaptation to gradually increased pulp density of arsenic-bearing refractory gold ore and concentration of arsenic by serial sub-culturing for about two years, its tolerance ability was up to 200 and 8 g/L, respectively. After that, the consortium was employed to treat arsenic-bearing refractory gold ore to evaluate its ability and potential for industrial application. The results indicated that the maximum cell density was 2′109 mL-1 (the 14th day) during the whole process and arsenic extraction rate reached up to 59.93% in 22 d. Microbial diversity monitored by restriction fragment length polymorphism suggested that the consortium mainly consisted of four species, Sulfobacillus sp., Acidithiobacillus caldus, Ferroplasma thermophilum and Pseudomonas sp.

Key words: refractory gold ore, stirred tank reactor, moderate thermophile, pretreatment, restriction fragment length polymorphism

摘要： 从多个矿坑、煤堆废水中富集中度嗜热浸矿菌，并在搅拌反应器中经过两年的长期驯化，获得能耐受高矿浆浓度和具有抗砷性的浸矿混合菌. 对浸矿混合菌进行耐高矿浆浓度的驯化，保持矿浆浓度为200 g/L，改变两种金矿的配比，提高驯化体系中砷含量，进行耐砷性驯化. 用驯化的浸矿混合菌对难处理金矿进行生物氧化预处理，矿浆浓度为200 g/L，砷含量为8 g/L，14 d时菌体浓度最大，达2′109 mL-1，22 d时砷浸出率为59.93%. 采用限制性酶切片段长度多态性分析对浸矿14和22 d时的微生物多样性进行分析，发现存在Sulfobacillus sp., Acidithiobacillus caldus, Ferroplasma thermophilum和Pseudomonas sp..

关键词: 难处理金矿, 搅拌槽反应器, 中度嗜热浸矿菌, 预处理, 限制性酶切片段长度多态性分析