Abstract: An elemental balance approach between culture medium and biomass composition is used to redesign BG11 culture medium, the main nutrients in the modified culture medium are KH2PO4 32 mg/L, MgSO4×7H2O 32 mg/L, (NH4)2SO4 22.4 mg/L, CaCl2×7H2O 8 mg/L, and FeSO4 2.6 mg/L. The results show that Scenedesmus dimorphus almost has the same initial growth curve in the modified and BG11 culture media, and the final volumetric yield of lipid is 0.407 and 0.403 g/L, respectively, in the case of reducing the cultivation cost to 33%~50%, Scenedesmus dimorphus in the modified and BG11 culture media almost obtains the same lipid yield.

Key words: Scenedesmus dimorphus, elemental balancing, culture medium, lipid yield

摘要： 根据微藻细胞营养元素含量对BG11培养基重新设计，改良培养基中主要营养盐浓度为(mg/L): KH2PO4 32, MgSO4·7H2O 32, (NH4)2SO4 22.4, CaCl2·7H2O 8, FeSO4 2.6. 培养实验结果显示，改良培养基和BG11培养基中二形栅藻的初始生长状态几乎相同，最终油脂产量分别为0.407和0.403 g/L，改良培养基的培养成本为BG11培养基的33%~50%.

关键词: 二形栅藻, 元素平衡, 培养基, 油脂产量