Abstract: Based on each stage of the protein expression process, current regulation strategies of the expression rate of transcription, translation and folding of heterologous proteins are introduced and evaluated respectively. Based on the fact that these strategies have different action stages, features, advantages and disadvantages, the idea of combining such strategies to respectively regulate each step of protein expression including transcription, translation and folding is proposed in order to obtain an appropriate overall expression rate and optimize the effect of expression, so as to improve the production efficiency and product quality of heterologous proteins in E. coli system and further its applicability and economy.

Key words: Escherichia coli, protein expression rate, regulation strategy, heterologous protein

摘要： 介绍和评价了目前针对大肠杆菌体系外源蛋白表达的转录、翻译和折叠阶段的各种表达速度调控策略. 根据现有各类调控策略的作用阶段、特点及优缺点，提出对蛋白表达各步骤分别调节，从而获得合适的整体表达速度以优化表达效果，提高大肠杆菌体系外源蛋白生产效率和产品质量，进一步提高其适用性和经济性.

关键词: 大肠杆菌, 蛋白表达速度, 调控策略, 外源蛋白