Abstract: Microalgae oil was extracted by six solvents commonly used for oil extraction, respectively, with ice bath ultrasonication for 15 min and extraction 8 h. The extraction behavior of microalgae oil with different solvents was studied. The results showed that for extraction of the oil from microalgae powder, n-hexane showed a better effect, resulting in a higher extraction rate of 71.52%, the oil contained 75.86% neutral lipid and 1.63% vitamin E, and the residual rates of protein, chlorophyll and carbohydrate were 55.92%, 61.33% and 78.35%, respectively. For extraction of the oil from microalgae ooze, n-hexane/ethanol mixed solvent showed a better effect, resulting in a higher extraction rate of 68.31%, the oil contained 71.65% neutral lipid and 1.87% vitamin E, and the residual rates of protein, chlorophyll and carbohydrate were 60.56%, 53.27% and 80.20%, respectively.

Key words: microalgae, oil, extraction, solvent, neutral lipid, vitamin E, residue

摘要： 分别向微藻中加入6种常用溶剂，冰浴超声处理15 min，振荡浸提8 h，提取微藻中的油脂，考察了溶剂的浸提能力. 结果表明，浸提干藻粉与湿藻泥中的油脂可选择不同溶剂，用正己烷浸提干藻粉中油脂，提取率可达71.52%，其中中性脂占75.86%，维生素含量E为1.63%，提取油脂后的藻渣中蛋白质、叶绿素和糖类的保留率分别为55.92%, 61.33%和78.35%，营养成分保留率较高；用正己烷/乙醇混合溶剂浸提湿藻泥中油脂，提取率可达68.31%，其中中性脂占71.65%，维生素E含量为1.87%，提取油脂后的藻渣中蛋白质、叶绿素和糖类的保留率分别为60.56%, 53.27%和80.20%.

关键词: 微藻, 油脂, 浸提, 溶剂, 中性脂, 维生素E, 藻渣