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Study on humic acid-like components, molecular structure and physiological activity
Bingkai SHANG Haitao MO Zhuowang FU Xiaoyong ZHANG
The Chinese Journal of Process Engineering
2021, 21 (8):
969-975.
DOI: 10.12034/j.issn.1009-606X.220291
By means of methods of elemental analysis, FT-IR and solid 13C NMR, a comparative study on the component, elemental composition and molecular structure of humic acid-like substances prepared from four different raw materials and humic acids from mineral sources were carried out. Also the physiological activity of four humic acid-like substances was determined by seed germination experiment. The study results showed that the content of fulvic acid in the humic acid-like materials prepared from herbaceous plants (wheat straw and bamboo) was significantly higher than that from woody plants (pine and poplar). The molecular structure of humic acid-like materials was similar to that of mineral humic acid, because both of them had the characteristic functional groups of humic acid. Compared with mineral humic acids, the structures of humic acid-like materials were more complex, and the types and contents of functional groups were different. They have more aromatic structures and lower oxygen-containing functional groups. Also, humic acid-like materials from herbaceous plants had higher aromatization degree and lower molecular weight than those from woody plants. The results of wheat seed germination experiment showed that, when the concentration of the humic acid-like materials was 20~40 mg/L, it can obviously promote the germination of seeds. However the seed growth will be inhibited if the concentration was too high.
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