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过程工程学报 ›› 2020, Vol. 20 ›› Issue (9): 1106-1113.DOI: 10.12034/j.issn.1009-606X.219312

• 生化工程 • 上一篇    下一篇

聚乙烯醇/牛I型胶原支架材料的制备及评价

孟 迪1， 雷雄心2， 李 阳2,3， 黄大卫1*， 张贵锋2*   

  1. 1. 南开大学生命科学学院，天津 300071 2. 中国科学院过程工程研究所，北京 100190 3. 南方医科大学珠江医院再生医学研究所，广东 广州 510282
  • 收稿日期:2019-09-29 修回日期:2020-01-03 出版日期:2020-09-22 发布日期:2020-09-23
  • 通讯作者: 张贵锋 gfzhang@ipe.ac.cn
  • 基金资助:
    广东省科技计划项目

Preparation and evaluation of polyvinyl alcohol/bovine type I collagen scaffolds

Di MENG1, Xiongxin LEI2, Yang LI2,3, Dawei HUANG1*, Guifeng ZHANG2*   

  1. 1. College of Life Sciences, Nankai University, Tianjin 300071, China 2. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 3. Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China
  • Received:2019-09-29 Revised:2020-01-03 Online:2020-09-22 Published:2020-09-23
  • Contact: ZHANG Gui-feng gfzhang@ipe.ac.cn

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摘要： 研究了一种聚乙烯醇(PVA)和胶原(COL)复合支架材料的制备方法。采用氨基硅烷对PVA海绵表面进行了氨基化修饰后，通过戊二醛溶液交联牛Ⅰ型胶原(COL)，最后通过赖氨酸溶液封闭，获得一种PVA/COL复合支架材料。采用扫描电镜(SEM)、X光电子能谱仪(XPS)、傅里叶红外光谱(FT-IR)等手段对支架材料的理化性能进行表征，并通过细胞实验对支架材料的生物学性能进行评价。结果表明，经过COL修饰的PVA孔隙率为21.33%，平均孔径为168.68 ?m且均匀分布，支架材料接触角为20.03°。对支架材料的生物学评价结果表明C3A细胞在复合材料上黏附良好，优于PVA组；CCK-8增殖检测结果表明细胞在复合材料上呈增殖生长趋势，与对照组PVA相比差异显著(P?0.01)。将PVA和COL复合制备得到的支架材料具有良好的理化及生物学特性，具有广阔的应用前景。

关键词: I型胶原, PVA, 支架材料, 肝细胞

Abstract: Collagen is an excellent biomedical material with biocompatibility and biodegradability. However, the poor stability and low mechanical strength of collagen limit its application. Polyvinyl alcohol has good physical and chemical properties which can form a good bio-scaffold material by chemically coupling with collagen. Bioartificial liver (BAL) support has been researched and widely used in providing a bridge to patients waiting for a liver transplant or promoting liver regeneration, which can prevent complications caused by liver failure and improve survival rate. The scaffolds of BAL are important for the reproduction and biological function of liver cells. Polyvinyl alcohol (PVA) and collagen (COL) composite scaffolds was prepared in this work. PVA was modified by the amino silane and collagen crosslinked by glutaraldehyde. Then the heterogeneous mixture was treated by lysine and freeze-dried to obtain the PVA/COL composite scaffolds. The physical and chemical properties of the scaffolds were analyzed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR). The biological properties of the scaffolds were evaluated by laser confocal, scanning electron microscopy (SEM) and CCK-8 experiments. FT-IR and XPS experiments demonstrated that collagen was successfully coupled to the PVA sponge and maintained a good triple helix structure. The results showed that the pore diameter of PVA modified by COL had uniform pore distribution with porosity of 21.33% and pore diameter of 168.68 μm. The contact angle of the scaffold material was 20.03°. The biological evaluation of scaffold materials showed that C3A cells adhered well on the PVA/COL scaffold and the proliferation test showed that the cells grew well on the composites. The proliferation of cells on the composite scaffolds was significantly different from the control group (P?0.01). The scaffolds prepared by the combination of PVA and COL had a promising application with the good physical and chemical properties.

Key words: type I collagen, polyvinyl alcohol, scaffold, C3A cells