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›› 2001, Vol. 1 ›› Issue (4): 0-0.

• 4 •    

温度诱导双水相分离纯化细菌素工艺条件的选取与优化

李孱，蔡昭铃, 丛威, 白景华, 欧阳藩   

  1. 1. 中国科学院化工冶金研究所生化工程国家重点实验室, 北京 100080; 2. 湖北大学生命科学院, 湖北 武汉 430062
  • 出版日期:2001-08-20 发布日期:2001-08-20

Silica Nanofibers Enwrapped with Well-organized AgCl Nanoparticles

LI Yong-jun, LIU Chun-yan, ZHANG Zhi-ying   

  1. 1. State Key Lab. Biochem. Eng., Inst. Chem. Metall., Chinese Academy of Sciences, Beijing 100080, China; 2. College of Life Science, Hubei University, Wuhan, Hubei 430062, China
  • Online:2001-08-20 Published:2001-08-20

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被引次数

52

摘要： 利用TritonX-114可萃取疏水性蛋白质的性质，研究了温度诱导双水相分离纯化细菌素乳链菌肽, 用响应面法对成相成份TritonX-114和离子强度进行了优化，最佳浓度分别为3.27% TritonX-114，0.85 mol/L氯化钠. 优化过程中，pH固定为2.0，温度固定为30℃, 在此条件下乳链菌肽的萃取率为92%，纯化系数为9.05.

关键词: 温度诱导双水相系统, 萃取, 细菌素, 响应面法, 优化

Abstract: Bacteriocin nisin was isolated and purified in temperature-induced aqueous two-phase systems using the capacity of extracting hydrophobic proteins of TritonX-114. The concentrations of sodium chloride and TritonX-114 which can form the aqueous two-phase systems were optimized by the response surface method. The full-factorial design was used to assess the main effects of factors and determine the path of steepest ascent to the optimal region of the aqueous two-phase system composition. The optimum concentrations of TritonX-114 and sodium chloride were then easily determined by using a central composite design and were found to be 3.27% of TritonX-114 and 0.85 mol/L sodium chloride. The recovery and purification factor were 92% and 9.05 respectively under the optimum conditions.

Key words: silver chloride, silica, nanofibers, nanoparticles, static self-assembly

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