Preparation of Ultrafine Silica Particles and Their Application in a-Amylase Immobilization
CAO Li-xia WANG Ling-ling LIU Hui-zhou YANG Chuan-fang
Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences Institute of Process Engineering, Chinese Academy of Sciences Institute of Process Engineering, Chinese Academy of Sciences
Abstract：50 nm and 1 mm SiO2 particles were prepared using TritonX-l00 reverse micellar system and St?ber method, respectively. The particles were then modified with dichlorodimethylsilane to render their surfaces hydrophobic for immobilization of a-amylase. The results showed that compared with unmodified SiO2 particles, the adsorption capacity of a-amylase on modified SiO2 particles was obviously improved, and the stability of immobilized a-amylase was also effectively increased, as evidenced by only 30% loss of activity after 6 times washing. The adsorption capacity of a-amylase on 1 μm SiO2 particles was higher than that on 50 nm SiO2 particles, while the activity of immobilized α-amylase on the different carriers was ranked in the order of 50 nm SiO2 > 1 mm SiO2 > modified 50 nm SiO2 > modified 1 mm SiO2.
曹丽霞 王玲玲 刘会洲 杨传芳. 超细SiO2颗粒的制备及其对a-淀粉酶的吸附固定化[J]. , 2015, 15(2): 336-340.
CAO Li-xia WANG Ling-ling LIU Hui-zhou YANG Chuan-fang. Preparation of Ultrafine Silica Particles and Their Application in a-Amylase Immobilization. , 2015, 15(2): 336-340.
2015, Vol. 15 
