Preparation of Uniform-sized pH-sensitive HTCC Hydrogel Microspheres by Premix Membrane Emulsification and Thermal Gelation
LIU Yan WANG Yue-qi FAN Qing-ze WU You-bin WU Jie MA Guang-hui
University of Chinese Academy of Sciences University of Chinese Academy of Sciences University of Chinese Academy of Sciences University of Chinese Academy of Sciences National Engineering Research Center for Biotechnology State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract：N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) hydrogel microspheres were prepared by premix membrane emulsification and thermal gelation methods, using HTCC/a-b-glycerophosphate mixed system with the property of thermal gelation as water phase. The effects of preparation conditions on size distribution, structure and drug encapsulation efficiency of microspheres, including transmembrane pressure, formulation of water phase and oil phase, volume ratio of water to oil phase and pore size of microporous membrane, were examined. After optimization, uniform-sized microspheres (polydispersity, PDI<0.1) with different sizes and high drug encapsulation efficiency (75.49%±2.62%) were obtained. The cytotoxicity and pH-sensitivity of as-obtained hydrogel microspheres were evaluated in comparison with the microspheres prepared by traditional method. The results showed that the as-obtained hydrogel microspheres possessed good biocompatibility and outstanding pH-sensitivity, and remained stable in neutral/alkalescent media and released drug in a relatively slow manner, with the drug cumulative release rate of 34.6% at 24 h in buffer solution at pH=7.4. While the hydrogel microspheres collapsed in acidic solution and led to a repaid drug release, and the cumulative release rate reached up to 79.6% in 1 h in buffer solution at pH=5.5.
刘艳 王月琦 范清泽 吴有斌 吴颉 马光辉. 快速膜乳化与热固化法制备粒径均一的pH敏感壳聚糖季铵盐凝胶微球[J]. 过程工程学报, 2015, 15(3): 473-481.
LIU Yan WANG Yue-qi FAN Qing-ze WU You-bin WU Jie MA Guang-hui. Preparation of Uniform-sized pH-sensitive HTCC Hydrogel Microspheres by Premix Membrane Emulsification and Thermal Gelation. Chin. J. Process Eng., 2015, 15(3): 473-481.
2015, Vol. 15 
