Separation of Propionibacterium freudenreichii Cells with Membranes and ex-situ Transformation of Vitamin B12
XU Guo-xia DU Wei WANG Zi-qiang WANG Yun-shan， ZHANG Li-ping SU Zhi-guo
(1.College of Life Science, Hebei University (1.College of Life Science, Hebei University Key Lab of Biochemical Engineering, Institute of Process Engineering, State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences College of Life Science, Hebei University State Key Lab. Biochem. Eng., Inst. Process Eng., Chinese Academy of Sciences
Abstract：The application of membrane separation of Propionibacterium freudenreichii cells was studied. The effects of membrane material and pore size on the flux and cellular activity were examined. The Vitamin B12 ex-situ transformation was also investigated. The results showed that spiral wound membrane was more suitable for on-line cell separation compared with ceramic membrane. When the fermentation broth was treated with 0.22 mm spiral wound membrane, the biological activity of cells was almost not damaged, with a survival rate of 99.5%, Abo-cbi conversion rate of 78.8% was obtained at 30℃ for 72 h with 4 times concentrated fermentation broth and the productivity of Vitamin B12 reached 53.9 mg/L.
徐国霞 杜崴 王自强 王云山 张利平 苏志国. 细胞膜分离费氏丙酸杆菌离位转化合成维生素B12[J]. 过程工程学报, 2016, 16(2): 298-302.
XU Guo-xia DU Wei WANG Zi-qiang WANG Yun-shan; ZHANG Li-ping SU Zhi-guo. Separation of Propionibacterium freudenreichii Cells with Membranes and ex-situ Transformation of Vitamin B12. Chin. J. Process Eng., 2016, 16(2): 298-302.
2016, Vol. 16 
