Preparation of Pluronic-based Magnetic Nanoparticles and Their Application in Purification of Laccase
LIU Xing-hua GUO Chen YANG Liang-rong WANG Feng LIU Chun-zhao LIU Hui-zhou
Key Lab. Green Process & Eng., Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering Institute of Process Engineering, Chinese Academy of Sciences
Abstract： Nano-sized monodispersed Pluronic (PEO-PPO-PEO)-based magnetic nanoparticles (PMNPs) were synthesized as carriers for the purification of laccase from the mycelium culture of Trametes versicolor. The adsorption assay was carried out in model system of laccase. The result showed that the maximum adsorption capacity was 0.535 mg/mg without any decrease in the range from 5℃ to 40℃. Purification of laccase from mycelium culture by PMNPs was also investigated. Total enzyme activity recovery rate could reach 62.9% with a single step, and purification fold 3.4.
刘兴华 郭晨 杨良嵘 王锋 刘春朝 刘会洲. Pluronic嵌段共聚物磁性纳米颗粒的制备及其对漆酶的分离纯化[J]. , 2009, 9(3): 456-461.
LIU Xing-hua GUO Chen YANG Liang-rong WANG Feng LIU Chun-zhao LIU Hui-zhou. Preparation of Pluronic-based Magnetic Nanoparticles and Their Application in Purification of Laccase. , 2009, 9(3): 456-461.
2009, Vol. 9 
