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›› 2003, Vol. 3 ›› Issue (5): 0-0.

• 5 •    

实验设计法优化核酸酶P1的发酵培养基

徐正军，肖林平，吕浩，谢宁昌，应汉杰   

  1. 南京工业大学制药与生命科学学院，江苏 南京 210009
  • 出版日期:2003-10-20 发布日期:2003-10-20

Application of Experimental Design to Optimizing the Medium of Nuclease P1

XU Zheng-jun, XIAO Lin-ping,LU Hao,XIE Ning-chang, YING Han-jie   

  1. Col. Life Sci. & Pharmaceut. Eng., Nanjing Univ. Technol., Nanjing, Jiangsu 210009, China
  • Online:2003-10-20 Published:2003-10-20

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摘要： 采用实验设计法研究了碳源、氮源和磷源等因素对桔青霉(Penicillium citrinum)M02发酵产核酸酶P1的影响. 实验结果表明，含有玉米浆的复合氮源可以明显地提高核酸酶P1的产量. 同时通过两轮实验建立了一个可以较好预测实际发酵的二次模型，并依据此模型优化了碳源、氮源以及磷源的组成，优化后的产核酸酶P1的发酵培养基组成为(g/L)：葡萄糖38.73，蛋白胨1.91，玉米浆1.84, KH2PO4 0.6, K2HPO4×3H2O 0.6, MgSO4 0.4, CaCl2 0.4, ZnSO4×7H2O 0.4. 用此培养基进行发酵，实际产酶水平为648.3 U/ml，与优化前的380 U/ml相比提高了约70%. 此外，还初步探讨了玉米浆促进P1酶发酵的机理，这是因为玉米浆与蛋白胨相比含有了较多有利于P1酶发酵的氨基酸，如甘氨酸、丙氨酸以及丝氨酸等.

关键词: 桔青霉M02, 核酸酶P1, 发酵培养基, 玉米浆, 实验设计, 优化

Abstract: Experimental design was employed to study the effects of several factors on the production of nuclease P1 by Penicillium citrinum M02, such as carbon source, nitrogen source, phosphor source and corn steep liquor which acted as complementary nitrogen source. The results of two experiments showed that corn steep liquor could remarkably promote the production of nuclease P1. In addition, one model that can describe the fermentation perfectly was gained from these experiments. When nuclease P1 was produced on the optimized medium calculated from this model, its yield was 648.3 U/ml that is comparable with 661 U/ml from the model. And it is improved by 70% compared with the 380 U/ml, which was gained on the original medium at the same time. The possible reason for corn steep liquor promoting nuclease P1 fermentation is that corn steep liquor contains more useful amino acids such as glycin, alanine, serine and so on.

Key words: Penicillium citrinum M02, nuclease P1, fermentation medium, corn steep liquor, experimental design, optimization

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