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过程工程学报 ›› 2023, Vol. 23 ›› Issue (4): 523-533.DOI: 10.12034/j.issn.1009-606X.222144

• 研究论文 • 上一篇    下一篇

基于正交法及Kriging模型的生物反应器搅拌桨优化设计

李树勋1,2， 马廷前1,2*， 胡迎港1,2， 于孟瑶1,2， 刘斌才1,2   

  1. 1. 兰州理工大学石油化工学院，甘肃 兰州 730050 2. 机械工业泵及特殊阀门工程研究中心，甘肃 兰州 730050
  • 收稿日期:2022-04-24 修回日期:2022-06-04 出版日期:2023-04-28 发布日期:2023-05-04
  • 通讯作者: 马廷前 mtqlut@163.com
  • 作者简介:李树勋，教授，化工过程机械专业，E-mail: gdlishuxun@126.com；通讯联系人，马廷前，硕士研究生，化工机械专业，E-mail: mtqlut@163.com

Optimization design of bioreactor agitator blade based on orthogonal method and Kriging model

Shuxun LI1,2,  Tingqian MA1,2*,  Yinggang HU1,2,  Mengyao YU1,2,  Bincai LIU1,2   

  1. 1. School of Petrochemical Technology, Lanzhou University of Technology, Lanzhou, Gansu 730050, China 2. Machinery Industry Pump and Special Valve Engineering Research Center, Lanzhou, Gansu 730050, China
  • Received:2022-04-24 Revised:2022-06-04 Online:2023-04-28 Published:2023-05-04

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摘要： 针对搅拌桨标准中设计参数区间大，生物反应器搅拌桨设计时参数难以确定、剪切力及搅拌功率缺少参考依据的问题。基于正交法及CFD对200 L生物反应器搅拌桨进行优化，由极差分析得到三斜叶桨不同搅拌桨直径、桨叶宽度、搅拌桨中心离底距离和转速对桨叶剪切速率和搅拌功率的影响规律，并结合流场分析选择有利于细胞培养的设计方案。根据正交优化方案确定多目标优化变量区间，以剪切力和搅拌功率为优化目标，基于Kriging模型和多目标遗传算法提出了有利于细胞培养的三斜叶桨设计变量区间，并优化得到桨叶平均剪切力为0.789 Pa、搅拌功率为0.395 W的搅拌桨最优参数组合，为生物反应器不同实际需求下的设计、优化提供了方法及数据参考。

关键词: 搅拌桨, 生物反应器, 优化设计, 正交法, Kriging模型

Abstract: The stirred bioreactor is widely used in cell culture in the biopharmaceutical industry, but there is a lack of research on optimization of agitator blade in bioreactor based on the standard of agitator blade and the actual demand. Aiming at the problems that the design parameters interval in the agitator blade standard is large, the parameters of the bioreactor stirring paddle design are difficult to determine, and the shear force and power consumption lack reference in the design of the agitator blade. The agitator blade of the 200 L bioreactor was preliminarily optimized based on the orthogonal method and CFD, and the effects of different agitator blade diameters, agitator blade width, distance from agitator blade center to bottom, and rotational speed on agitator blade shear rate and agitation power of the tri-oblique blade were obtained by using the method of range analysis. Combined with the velocity dead zone distribution, streamline distribution, and shear force distribution, a design scheme that ensured the effect of cell culture was selected. This scheme was conducive to fluid circulation and could promote the mixing of culture medium and gas-liquid mass transfer. At the same time, the fluid needed sufficient kinetic energy and overall flow velocity. According to the orthogonal optimization scheme, the multi-objective optimization variables interval was determined, and the shear force and power consumption were taken as the optimization objectives. Based on the Kriging model and multi-objective genetic algorithm, the design variables interval of the tri-oblique blade propeller that is beneficial to cell culture was proposed. The optimal parameter combination of the agitator blade with average shear force of 0.789 Pa and power consumption of 0.395 W was obtained, which provided a method and data reference for the design and optimization of the bioreactor under different actual needs.

Key words: agitator blade, bioreactor, optimization design, orthogonal method, Kriging model