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›› 2008, Vol. 8 ›› Issue (6): 1200-1203.

• 生化工程专栏 • 上一篇    下一篇

氨基酸强化Torulopsis glabrata发酵生产丙酮酸

许庆龙 许小鹏 刘立明 史仲平 堵国成 陈坚   

  1. 江南大学 江南大学工业生物技术教育部重点实验室 江南大学生物工程学院环境生物技术实验室 江南大学生物工程学院 江南大学工业生物技术教育部重点实验室 江南大学工业生物技术教育部重点实验室
  • 收稿日期:2008-05-09 修回日期:2008-07-31 出版日期:2008-12-20 发布日期:2008-12-20
  • 通讯作者: 陈坚

Enhancement of Pyruvate Production with Amino Acid Addition in Torulopsis glabrata Induced Fermentation

XU Qing-long XU Xiao-peng LIU Li-ming SHI Zhong-ping DU GUo-cheng CHEN Jian   

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University Lab of Biotechnology, School of Biotechnology, Southern Yangtze University Lab of Environmental Biotechnology, School of biotechnology Southern Yangtze University Key Lab. Industrial Biotechnol., Ministry of Education, Jiangnan University
  • Received:2008-05-09 Revised:2008-07-31 Online:2008-12-20 Published:2008-12-20
  • Contact: CHEN Jian

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摘要： 在维生素限量添加的条件下，研究了添加氨基酸对丙酮酸发酵的影响. 在发酵初始添加0.8 g/L谷氨酸、0.6 g/L酪氨酸和0.2 g/L甲硫氨酸使丙酮酸产量分别提高23.5%, 16.4%和11.8%. 动力学分析表明，添加以上3种原料提高丙酮酸生产性能的原因在于用于细胞生长的葡萄糖下降，显著提高了细胞对葡萄糖的产率系数(Yx/s)和丙酮酸对葡萄糖的产率系数(Yp/s). 添加氨基酸导致胞内NADH/NAD+比下降，增加了胞内NAD+可用度，加强了糖酵解速度，从而提高了丙酮酸生产强度.

关键词: 光滑球拟酵母, 氨基酸, 丙酮酸, 发酵

Abstract: The effect of amino acid on production of pyruvate by T. glabrata CCTCC M202019 was investigated. The pyruvate production was increased 23.5%, 16.4% and 11.8%, respectively, with 0.8 g/L glutamic acid, 0.6 g/L tyrosine and 0.2 g/L methionine fed to the fermentation medium at the initial fermentation. The principle of amino acids to enhance pyruvate production is that the amount of glucose used for cell growth was reduced, but the yields of cell and pyruvate on glucose (Yx/s and Yp/s) were increased. On the other hand, the concentration of intracellular NADH was decreased by amino acid addition, which resulted in higher glycolic flux and pyruvate production.

Key words: Torulopsis glabrata, amino acid, pyruvate, fermentation