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过程工程学报 ›› 2016, Vol. 16 ›› Issue (1): 151-155.DOI: 10.12034/j.issn.1009-606X.215317

• 生化工程专栏 • 上一篇    下一篇

螺旋藻回用培养液组成对藻细胞生长的影响

于水淼 贺乐 吴霞 颜成虎 汪建明 丛威 卢运明   

  1. 天津科技大学食品工程与生物技术学院 中科院过程工程研究所生化工程国家重点实验室 中科院过程工程研究所生化工程国家重点实验室 天津科技大学食品工程与生物技术学院 中国科学院过程工程研究所生化工程国家重点实验室 深圳市绿得宝保健食品有限公司
  • 收稿日期:2015-08-28 修回日期:2015-10-13 出版日期:2016-02-20 发布日期:2016-12-22
  • 通讯作者: 于水淼

Effect of Composition of Spirulina platensis Reused Culture Medium on the Growth of Its Cells

YU Shui-miao HE Le WU Xia YAN Cheng-hu WANG Jian-ming CONG Wei LU Yun-ming   

  1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Acadeny of Sciences State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Acadeny of Sciences College of Food Engineering and Biotechnology, Tianjin University of Science and Technology State Key Lab. Biochem. Eng., Inst. Process Eng., CAS State Key Lab. Biochem.Shenzhen Lvdebao Health Food Limited Company
  • Received:2015-08-28 Revised:2015-10-13 Online:2016-02-20 Published:2016-12-22
  • Contact: YU Shui-miao

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摘要： 将螺旋藻回用培养液补齐营养盐用于培养螺旋藻，考察了螺旋藻生物量、培养液中硝酸盐和磷酸盐及培养末期螺旋藻胞内生化成分含量对藻细胞生长的影响；用超滤膜对回用培养液中物质进行分子量分级，分析各级组分对螺旋藻的抑制效应，确定抑制物的分子量分布范围，并分析其成分. 结果表明，回用培养液中的螺旋藻胞外分泌物对藻细胞生长有明显抑制作用，比生长速率比新鲜培养基中低23%，且会抑制藻细胞对营养盐的吸收及胞内蛋白和叶绿素合成，对硝酸盐、磷酸盐的吸收分别低36%和37%，胞内蛋白及叶绿素含量分别为新鲜培养基中的0.85和0.65倍. 抑制物为分子量大于100 kDa的螺旋藻胞外多糖.

关键词: 螺旋藻, 回用培养液, 生长抑制物, 胞外多糖, 硝酸盐, 磷酸盐

Abstract: A long-term reused culture solution of Spirulina platensis was added with nutrient salt for cultivation of itself. The effects of cell density, concentrations of phosphate and nitrate, and contents of biological components in it in its final cultivation on the growth of itself were examined. The compounds in the solution were classified in molecular weight by ultrafiltration membranes. Their inhibitory activity on the cultivation was measured, and molecular weight distribution of inhibitors analyzed, and their compositions were determined. The results showed that the long-term reused culture medium inhibited the cell growth of Spirulina platensis, although nutrition was complemented enough, the specific growth rate was decreased by 23%, compared with fresh culture medium, and the absorption rate of nitrate and phosphate was decreased by 36% and 37%, as the metabolism of nitrogen and phosphorus and the synthesis of intracellular proteins and chlorophyll of Spirulina platensis were inhibited. The content of intracellular proteins was 0.85 of that of fresh culture medium, and the content of chlorophyll 0.65. The auto-growth inhibitor of Spirulina platensis was extracellular polysaccharide with the molecular weights over 100 kDa.

Key words: Spirulina platensis, reused culture medium, growth inhibitor, extracellular polysaccharide, nitrate, phosphate

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