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›› 2005, Vol. 5 ›› Issue (4): 438-441.

• 生化工程专栏 • 上一篇    下一篇

通过氮浓度调节塔玛亚历山大藻毒素产量的初步研究

胡晗华,石岩峻,丛威   

  1. 中国科学院水生生物研究所
  • 出版日期:2005-08-20 发布日期:2005-08-20

A Preliminary Study of Improvement in Toxin Production of Alexandrium tamarense by Regulation of Nitrate Concentrations

HU Han-hua,SHI Yan-jun,CONG　Wei   

  1. Institute of Hydrobiology, Chinese Academy of Sciences
  • Online:2005-08-20 Published:2005-08-20

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摘要： 初步研究了培养基中氮浓度及补加氮对塔玛亚历山大藻毒素产量的影响. 低氮(88.2 mmol/L)条件下藻细胞具有较高的比生长速率(0.60 d-1)，是高氮(882 mmol/L)下的约1.2倍. 低氮下在藻细胞对数生长的末期(第10天)补加氮(至高氮水平)导致塔玛亚历山大藻的最大细胞密度(48.5′103 mL-1)明显增加，分别是低氮(6.28′103 mL-1)和高氮(21′103 mL-1)下的7.7和2.3倍；最大单位细胞毒素含量(1.26 pg/cell)和产量(43.51 mg/L)分别是低氮(1.09 pg/cell和6.49 mg/L)和高氮(0.88 pg/cell和13.18 mg/L)下的1.2, 1.4倍和6.7, 3.3倍. 结果显示，在培养基中低水平氮、磷条件下塔玛亚历山大藻细胞的毒素合成增加，其中特别是磷限制条件下，藻细胞的毒素含量增加更为显著. 通过低氮下补加氮源以维持培养基中的低氮水平，可以显著促进塔玛亚历山大藻细胞的毒素产量.

关键词: 塔玛亚历山大藻, 贝类毒素, 氮, 磷

Abstract: The influence of nitrate concentration on toxin content of Alexandrium tamarense was investigated, which gave rise to the supply of an appropriate nutrition condition and culture technique of increasing the toxin production of Alexandrium tamarense. Low nitrate level (88.2 mmol/L) resulted in higher specific growth rate, which was 1.2 times of that at high nitrate level (882 mmol/L). When cultures at low nitrate level were supplemented with nitrate at 793.8 mmol/L in late exponential growth phase, the highest cell density (48.5′103 mL-1) was drastically enhanced, which was 7.7 and 2.3 times of those under low (6.28′103 mL-1) and high (21′103 mL-1) nitrate conditions, meanwhile the highest toxin content and production were 1.26 pg/cell and 43.51 mg/L, which were 1.2 and 1.4 or 6.7 and 3.3 times of those under low and high nitrate conditions respectively. The results showed that toxin content of Alexandrium tamarense was enhanced at low nitrate and phosphate levels, and in particular toxin content increased significantly under limited-phosphate conditions. The supplementation of nitrate under low nitrate condition to maintain low level of nitrate contributed to the effective increase of toxin production of Alexandrium tamarense.

Key words: Alexandrium tamarense, saxitoxin, nitrate, phosphate