氧化亚铁硫杆菌的分离培养及其浸磷效果

›› 2007, Vol. 7 ›› Issue (3): 584-588.

氧化亚铁硫杆菌的分离培养及其浸磷效果

龚文琪,陈伟,张晓峥,边勋,刘艳菊,刘俊,黄永炳,杨红刚

武汉理工大学资源与环境工程学院

出版日期:2007-06-20 发布日期:2007-06-20

Isolation and Cultivation of Acidithiobacillus ferrooxidans and Its Effects on Bioleaching of Phosphate Ore

GONG Wen-qi,CHEN Wei,ZHANG Xiao-zheng,BIAN-Xun,LIU Yan-ju,LIU Jun,HUANG Yong-bing,YANG Hong-gang

School of Resources and Environmental Engineering, Wuhan University of Technology

Online:2007-06-20 Published:2007-06-20

摘要/Abstract

摘要： 从安徽某煤矿的酸性矿坑水中分离出能有效浸出低品位磷矿的氧化亚铁硫杆菌菌株，对其形态特征和生长特性进行了初步的研究，并通过研究不同固体培养基成分对氧化亚铁硫杆菌分离纯化的影响，确定了适宜的分离条件：9K固体培养基的Fe2+浓度为4.5 g/L，采用下层涂布异养菌(红酵母菌)、上层涂布氧化亚铁硫杆菌的双层平板. 考察了培养基、能源物质及表面活性剂等因素对所选育的氧化亚铁硫杆菌的浸磷效果的影响，通过初步实验，浸磷率可达48%.

关键词: 氧化亚铁硫杆菌, 分离纯化, 固体培养基, 磷矿分解, 细菌浸出, 浸磷率

Abstract: A strain of Acidithiobacillus ferrooxidans that can be used effectively in the leaching of low grade phosphate ore was isolated from acid mine drainage from a coal mine in Anhui Province in China. Its characteristics of morphology and growth were studied. Through the investigation of the effects of different solid culture medium components on the isolation and purification of Acidthiobacillus ferrooxidans, the optimum isolation conditions were determined as: the concentration of Fe2+ in 9K solid culture medium was 4.5 g/L. A double-layer plate, with heterotrophic microbe Rhodotorula sp. spreading onto the bottom layer and chemoautotrophic bacterium At. f spreading onto the upper layer, was used. The influential factors including culture media, energy source materials and the addition of surfactant on the leaching of phosphate ore with the cultivated At. f were studied. Through preliminary experiments, up to 48% of phosphate in the ore was leached out using the isolated At. f strain.

Key words: Acidithiobacillus ferrooxidans, isolation and purification, solid culture medium, phosphate ore dissolution, bacterial leaching, phosphate leaching rate

龚文琪;陈伟;张晓峥;边勋;刘艳菊;刘俊;黄永炳;杨红刚. 氧化亚铁硫杆菌的分离培养及其浸磷效果[J]. , 2007, 7(3): 584-588.

GONG Wen-qi;CHEN Wei;ZHANG Xiao-zheng;BIAN-Xun;LIU Yan-ju;LIU Jun;HUANG Yong-bing;YANG Hong-gang. Isolation and Cultivation of Acidithiobacillus ferrooxidans and Its Effects on Bioleaching of Phosphate Ore[J]. , 2007, 7(3): 584-588.

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