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›› 2012, Vol. 12 ›› Issue (1): 44-48.

• 反应与分离 • 上一篇    下一篇

发酵液中乳酸的盐析萃取

魏搏超 宋志远 孙亚琴 修志龙   

  1. 大连理工大学生命科学与技术学院 大连理工大学生命科学与技术学院 大连理工大学环境与生命学院生物科学与工程系 大连理工大学生命科学与技术学院
  • 收稿日期:2011-07-22 修回日期:2012-01-10 出版日期:2012-02-20 发布日期:2012-02-20
  • 通讯作者: 魏搏超

Salting-out Extraction of Lactic Acid from Fermentation Broth

WEI Bo-chao SONG Zhi-yuan SUN Ya-qin XIU Zhi-long   

  1. School of Life Science and Biotechnology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology School of Life Science and Biotechnology, Dalian University of Technology
  • Received:2011-07-22 Revised:2012-01-10 Online:2012-02-20 Published:2012-02-20
  • Contact: WEI Bo-chao

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摘要： 研究了一种利用盐析萃取法分离发酵液中乳酸的新方法. 通过系统考察乳酸在不同盐析萃取体系中的分配规律，发现K2HPO4-甲醇和K2HPO4-乙醇体系适合分离发酵液中的乳酸. 发酵液中乳酸浓度为167 g/L时，采用25%(w) K2HPO4-26%(w)甲醇盐析萃取体系，乳酸的分配系数和回收率分别为4.01和86.0%；采用14%(w) K2HPO4-30%(w)乙醇盐析萃取体系，乳酸的分配系数和回收率分别为3.23和90.6%. 此时上相中残余葡萄糖、菌体和可溶性蛋白的去除率分别达67.3%, 100%和85.9%.

关键词: 乳酸, 盐析萃取, 发酵液

Abstract: Salting-out extraction was used for recovery of lactic acid from fermentation broth. The different hydrophilic organic solvents-inorganic salts systems were investigated for their extraction ability. The results showed that the K2HPO4-methanol and K2HPO4-ethanol systems appeared to be favorable. The partition coefficient and recovery rate of lactic acid reached up to 4.01 and 86.0% for the system of 25%(w) K2HPO4-26%(w) methanol, when the concentration of lactic acid was 167 g/L in the broth. For the system of 14%(w) K2HPO4-30%(w) ethanol, the partition coefficient and recovery rate of lactic acid reached up to 3.23 and 90.6%. The removal rate of glucose, cells and proteins reached 67.3%, 100% and 85.9%, respectively.

Key words: lactic acid, salting-out extraction, fermentation broth

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