基于发酵动力学模型的小球藻高密度发酵培养

doi:10.12034/j.issn.1009-606X.217318

过程工程学报 ›› 2018, Vol. 18 ›› Issue (3): 624-631.DOI: 10.12034/j.issn.1009-606X.217318

基于发酵动力学模型的小球藻高密度发酵培养

周有彩1，何勇锦1,2，李林声3，王明兹1,2，陈必链1,2*，郑行3

1. 福建师范大学生命科学学院，福建福州 350117；2. 福建师范大学工业微生物教育部工程研究中心，福建福州 350117； 3. 福清市新大泽螺旋藻有限公司，福建福清 350300

收稿日期:2017-09-01 修回日期:2017-10-24 出版日期:2018-06-22 发布日期:2018-06-06
通讯作者: 陈必链 chenbil@fjnu.edu.cn
基金资助:
“十三五”海洋经济创新发展示范项目子课题

High Cell Density Fermentation of Chlorella Based on Kinetics Model

Youcai ZHOU1, Yongjin HE1,2, Linsheng LI3, Mingzi WANG1,2, Bilian CHEN1,2*, Xing ZHENG3

1. School of Life Science, Fujian Normal University, Fuzhou, Fujian 350117, China; 2. Engineering Research Center of Industrial Microbiology, Ministry of Education, Fuzhou, Fujian 350117, China; 3. Fuqing King Dnarmsa Spriulina Co., Ltd., Fuqing, Fujian 350300, China

Received:2017-09-01 Revised:2017-10-24 Online:2018-06-22 Published:2018-06-06

摘要/Abstract

摘要： 构建了50 L发酵罐小球藻分批培养动力学模型，采用补料策略高密度发酵培养小球藻，考察了补料发酵过程中碳源的利用情况，采用实时荧光定量PCR技术分析了蛋白质合成关键酶二氨基庚二酸异构酶(dapF)、柠檬酸合成酶(CS)和葡萄糖?6-磷酸脱氢酶(G6PDH)的基因表达情况. 结果表明，小球藻经补料培养120 h，细胞生物量达106.65 g/L，平均生长速率为0.89 g/(L?h)，葡萄糖的细胞得率为0.56 g/g，发酵过程中葡萄糖和尿素浓度对小球藻的dspF, CS和G6PDH基因表达量有重要影响.

关键词: 小球藻, 发酵动力学, 补料培养, 高密度, 基因表达

Abstract: The kinetic model of batch fermentation of Chlorella sp. MBFJNU-17 in a 50 L fermenter was built, fed-batch strategy for high cell density cultivation was established. The application of carbon source was investigated. Furthermore, real-time quantitative PCR was used to determine the gene levels of key metabolic enzymes such as diaminopimelate isomerase (dapF), citrate synthase (CS) and glucose?6-phosphate dehydrogenase (G6PDH) in fed-batch fermentation. The results showed that the cell dry weight of Chlorella sp. MBFJNU-17 was 106.65 g/L, the average growth rate was 0.89 g/(L?h) and the yield of cell dry weight on glucose was 0.56 g/g, respectively, after fed-batch culture for 120 h. The gene levels of dapF, G6DPH and CS of Chlorella sp. MBFJNU-17 were strongly related with the concentrations of glucose and urea during the fermentation.

Key words: Chlorella sp. MBFJNU-17, Fermentation kinetics, Fed-batch culture, High cell density, Gene

周有彩何勇锦李林声王明兹陈必链郑行. 基于发酵动力学模型的小球藻高密度发酵培养[J]. 过程工程学报, 2018, 18(3): 624-631.

Youcai ZHOU Yongjin HE Linsheng LI Mingzi WANG Bilian CHEN Xing ZHENG. High Cell Density Fermentation of Chlorella Based on Kinetics Model[J]. Chin. J. Process Eng., 2018, 18(3): 624-631.

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基于发酵动力学模型的小球藻高密度发酵培养

High Cell Density Fermentation of Chlorella Based on Kinetics Model

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