• 生化工程 •

### 响应面法优化西兰花种子萝卜硫素前体脂质体的制备工艺

1. 1. 沈阳药科大学制药工程学院，辽宁 沈阳 110000 2. 中国科学院沈阳应用生态研究所，辽宁 沈阳 110000
• 收稿日期:2020-02-20 修回日期:2020-05-12 出版日期:2021-03-22 发布日期:2021-03-23
• 通讯作者: 韩静 921762467@qq.com
• 基金资助:
萝卜硫素纳米自微乳递送系统的研究与开发

### Optimization of preparation of sulforaphane proliposome in broccoli seeds by response surface method

Huanpu XU1, Zhaoyang PEI1, Shijie SUN1, Yingxue WU1, Lulu XU1, Jing HAN1*, Hui XU2*

1. 1. Shenyang Pharmaceutical University School of Pharmaceutical Engineering, Shenyang, Liaoning 110000, China 2. Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110000, China
• Received:2020-02-20 Revised:2020-05-12 Online:2021-03-22 Published:2021-03-23

Abstract: Sulforaphane is made into proliposomes to improve the stability of liposomes, while improving the water solubility and bioavailability of sulforaphane. Taking the encapsulation efficiency and particle size as indicators, the carrier materials, the type and amount of surfactants and the effects of lipid-drug ratios were investigated respectively. The optimal formulation of proliposome was optimized by response surface method. The stability of liposomes and proliposomes was investigated through room temperature stability experiments. The results showed that the optimal prescription was that the mass ratio of lipid phase to sulforaphane was 6.5:1, the mass ratio of NaCl to sulforaphane was 105:1, and the mass ratio of poloxamer-188 to sulforaphane was 1.5:1. The maximum average encapsulation rate was 77.43%, and the average particle size was 160.5 nm. Stability experiment results showed that when the drug retention rate was used as an indicator, liposomes and proliposomes had good stability within 60 days and a high retention rate of sulforaphane. When the encapsulation rate was used as an indicator, the liposome suspension produced precipitated, the encapsulation rate decreased, and the encapsulation rate of the proliposome did not decrease significantly within 60 days. This indicated that the proliposome can solve the oxidative deterioration of sulforaphane and the decrease of the encapsulation rate of the liposome due to precipitation, flocculation and other reasons. The proliposome has high encapsulation efficiency and simple preparation. It not only improves the stability of sulforaphane, but also improves the water solubility of sulforaphane and has broad application prospects.