Abstract: With the analysis technologies of HPLC, TLC and test of antibacterial bio-activity, the stability of maituolaimycin, a novel macrolide antibiotic, during the process of separation and purification from the fermentation broth was studied in orthogonal test. The research included pH, temperature and protein-removing, the main factors that affected the stability of maituolaimycin, as well as their interaction, and the authors put out a reasonable technological process forward the separation and purification of maituolaimycin. Also the optimized separation and purification process after fermentation offers an analogy for other antibiotics.

Key words: HPLC, maituolaimycin, separation, stability, TLC

摘要： 综合应用高效液相色谱HPLC、高效薄层色谱TLC技术、微生物抗菌活性检测方法，通过正交实验设计，对新型抗生素麦拓莱霉素在发酵液分离提纯过程中不同pH、温度、除蛋白工艺中的稳定性进行了研究. 考察了影响该抗生素稳定性的主要因素及其交互作用，提出了合理的抗生素分离纯化工艺流程，为相关抗生素的分离纯化及发酵液体系处理提供参考.

关键词: HPLC, 麦拓莱霉素, 分离, 稳定性, TLC