Abstract: In order to resolute racemic ibuprofen more effectively in organic solvent, a new method for the immobilization of Penicillium expansum lipase in macroporous resin AB-8 was established. The lipase, PEL, immobilized in macroporous resin AB-8 exhibited higher activity and enatioselectivity than others. The resolution of (R,S)-ibuprofen was performed in microaqueous media by PEL immobilized in macroporous resin AB-8. It was found that medium type, "memorial" pH value and water content were of great effects on the resolution of racemic ibuprofen, and the conversion rate of the reaction catalyzed by the lipase immobilized in macroporous resin AB-8 reached 47% with excellent eep (98.75%) in isooctane without water at "memorial" pH 9.0, 40℃ for 30 h. Immobilization by this method affected the enzymatic properties of PEL in nonaqueous reaction media. The immobilized PEL was then not easy to agglomerate in nonaqueous media. And its activity in the resolution reaction was enhanced accordingly. The immobilized lipase in the resolution reaction was stable and could be well maintained. And the conversion rate of the reaction catalyzed by immobilized PEL reached 47% and the eep was more than 98% with excellent enantioselectivity when the enzymatic resolution reached equilibrium after 30 h. So PEL immobilized in macroporous resin AB-8 had a potential use in the resolution of ibuprofen.

Key words: lipase, immobilization, ibuprofen, resolution

摘要： 为了提高扩展青霉TS414脂肪酶(PEL)对布洛芬的拆分效率，建立了适于非水相中选择性拆分(R,S)-布洛芬的固定化方法. 结果表明，固定化介质的类型、冻干pH和外加水量等因素对固定化PEL酶促拆分(R,S)-布洛芬有较大影响. 在冻干pH为9.0、外加水量为0、以大孔吸附树脂AB-8为固定化载体的体系中，40℃反应30 h后，拆分反应的转化率可达47%，对映体过量值eeP可达98.75%. AB-8固定化后，PEL在有机相反应体系中的分散性得到了明显改善，大幅度提高了酶促拆分反应的效率；大孔吸附树脂AB-8固定化PEL具有较高的操作稳定性，连续10批拆分反应的平均转化率在47%以上，eeP值均稳定在98%以上.

关键词: 脂肪酶, 固定化, 布洛芬, 拆分